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Immunohistochemistry (IHC) (Anti-SIX4 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Mouse anti-Human SIX4 Monoclonal Antibody | anti-SIX4 antibody

Mouse Monoclonal [clone 3B8] (IgG1,k) to Human SIX4

Gene Names
SIX4; AREC3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
SIX4; Monoclonal Antibody; Mouse Monoclonal [clone 3B8] (IgG1; k) to Human SIX4; Anti-SIX4 Antibody (clone 3B8) IHC-plus; AREC3; Homeobox protein SIX4; Sine oculis homeobox homolog 4; SIX homeobox 4; Human SIX4; anti-SIX4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3B8
Specificity
Human SIX4
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2
Concentration
0.41 mg/ml (varies by lot)
Sequence Length
781
Applicable Applications for anti-SIX4 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (10 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Target Species
Human
Immunogen Description
SIX4 (NP_059116, 672 a.a. ~ 781 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
SIX4 antibody was raised against sIX4 (NP_059116, 672 a.a. ~ 781 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SIX4 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-SIX4 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(SIX4 monoclonal antibody (M05), clone 3B8 Western blot of SIX4 expression in HeLa NE.)

Western Blot (WB) (SIX4 monoclonal antibody (M05), clone 3B8 Western blot of SIX4 expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SIX4 is approximately 0.1 ng/ml as a capture antibody.)

ELISA (EIA) (Detection limit for recombinant GST tagged SIX4 is approximately 0.1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,933 Da
NCBI Official Full Name
homeobox protein SIX4
NCBI Official Synonym Full Names
SIX homeobox 4
NCBI Official Symbol
SIX4
NCBI Official Synonym Symbols
AREC3
NCBI Protein Information
homeobox protein SIX4; sine oculis homeobox homolog 4
UniProt Protein Name
Homeobox protein SIX4
Protein Family
UniProt Gene Name
SIX4
UniProt Entry Name
SIX4_HUMAN

NCBI Description

This gene encodes a member of the homeobox family, subfamily SIX. The drosophila homolog is a nuclear homeoprotein required for eye development. Studies in mouse show that this gene product functions as a transcription factor, and may have a role in the differentiation or maturation of neuronal cells. [provided by RefSeq, May 2010]

Uniprot Description

SIX4: a member of the homeobox family, subfamily SIX. The drosophila homolog is a nuclear homeoprotein required for eye development. Studies in mouse show that this gene product functions as a transcription factor, and may have a role in the differentiation or maturation of neuronal cells. [provided by RefSeq, May 2010]

Protein type: Cell development/differentiation; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: tongue development; anatomical structure morphogenesis; skeletal muscle development; inner ear morphogenesis; pharyngeal system development; transcription, DNA-dependent; thymus development; positive regulation of transcription, DNA-dependent; male gonad development; embryonic cranial skeleton morphogenesis; sarcomere organization; olfactory placode formation; regulation of epithelial cell proliferation; male sex differentiation; regulation of protein localization; generation of neurons; male sex determination; myoblast migration; regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

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Product Notes

The SIX4 six4 (Catalog #AAA249098) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 3B8] (IgG1,k) to Human SIX4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIX4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (10 ug/ml), WB Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein. Researchers should empirically determine the suitability of the SIX4 six4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIX4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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