Rat anti-Human SIRT2 phospho Ser331 Monoclonal Antibody | anti-SIRT2 antibody
SIRT2 phospho Ser331 antibody (mAb)(Clone 6B5)
Background: SIRT2 (sirtuin 2) is a member of the SIR2/Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARg and subsequent repression of PPARg mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis. Ser-331 is phosphorylated by the cyclin kinases Cdk2 and Cdk5 and this phosphorylation inhibits SIRT2's catalytic activity.
NCBI and Uniprot Product Information
Observed: 52 kDa
NCBI Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
SIRT2: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Deacetylase; EC 3.5.1.-
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: microtubule; centrosome; lateral loop; paranode region of axon; perikaryon; nuclear heterochromatin; chromosome; cytosol; centriole; chromatin silencing complex; growth cone; perinuclear region of cytoplasm; cytoplasm; plasma membrane; spindle; midbody; nucleus; myelin sheath
Molecular Function: ubiquitin binding; zinc ion binding; histone deacetylase binding; protein deacetylase activity; beta-tubulin binding; transcription factor binding; histone acetyltransferase binding; protein binding; tubulin deacetylase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; NAD+ ADP-ribosyltransferase activity
Biological Process: chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of autophagy; phosphoinositide 3-kinase cascade; regulation of myelination; regulation of cell cycle; chromatin silencing; positive regulation of attachment of spindle microtubules to kinetochore; response to redox state; gene silencing; negative regulation of transcription from RNA polymerase II promoter; chromatin silencing at telomere; negative regulation of cell proliferation; substantia nigra development; meiotic cell cycle; protein amino acid deacetylation; cellular lipid catabolic process; protein kinase B signaling cascade; positive regulation of DNA binding; mitosis; regulation of phosphorylation; positive regulation of meiosis; transcription, DNA-dependent; regulation of exit from mitosis; hepatocyte growth factor receptor signaling pathway; histone deacetylation; negative regulation of striated muscle development; negative regulation of fat cell differentiation; myelination in the peripheral nervous system; protein amino acid ADP-ribosylation; cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell division; autophagy; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process
Research Articles on SIRT2
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Product Notes
The SIRT2 sirt2 (Catalog #AAA388864) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The SIRT2 phospho Ser331 antibody (mAb)(Clone 6B5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT2 phospho Ser331 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the SIRT2 sirt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT2 phospho Ser331, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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