Testing Data
(Figure 1. THP-1 cell line were surface stained with Rabbit anti-SIRPalpha monoclonal antibody 1ug/ml (clone: DM8) and Rabbit IgG isotype control antibody followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Rabbit anti-Human SIRPalpha Monoclonal Antibody | anti-SIRPalpha antibody
Anti-SIRPalpha (DM8) antibody, Rabbit mAb
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
SIRPalpha; Monoclonal Antibody; Anti-SIRPalpha (DM8) antibody; Rabbit mAb; BIT; CD172a; MFR; MYD-1; P84; PTPNS1; SHPS-1; SHPS1; SIRP; SIRP-ALPHA-1; SIRPalpha2; anti-SIRPalpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM8
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Liquid
Preservative: 0.1%Procline 300
Constituents: 50%Glycerol; PBS, pH7.4; 0.1%BSA
Preservative: 0.1%Procline 300
Constituents: 50%Glycerol; PBS, pH7.4; 0.1%BSA
Applicable Applications for anti-SIRPalpha antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
FC/FACS: 1:100
IP: 1:30
IP: 1:30
Immunogen
Recombinant human SIRPalpha (Glu31-Arg370) produced by using human HEK293 cells
Preparation and Storage
Store at -20 degree C for 15 months (Avoid repeated freezing and thawing)
Shipping: Ice Pack
Shipping: Ice Pack
Testing Data
(Figure 1. THP-1 cell line were surface stained with Rabbit anti-SIRPalpha monoclonal antibody 1ug/ml (clone: DM8) and Rabbit IgG isotype control antibody followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Testing Data
(Figure 1. THP-1 cell line were surface stained with Rabbit anti-SIRPalpha monoclonal antibody 1ug/ml (clone: DM8) and Rabbit IgG isotype control antibody followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Flow Cytometry (FC/FACS)
(Figure 2. Left) Flow cytometry data of serially titrated Rabbit anti-SIRPalpha monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. Right) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.)
Flow Cytometry (FC/FACS)
(Figure 2. Left) Flow cytometry data of serially titrated Rabbit anti-SIRPalpha monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. Right) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.)
Related Product Information for anti-SIRPalpha antibody
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
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Product Notes
The SIRPalpha (Catalog #AAA8579070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRPalpha (DM8) antibody, Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRPalpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF). FC/FACS: 1:100 IP: 1:30. Researchers should empirically determine the suitability of the SIRPalpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRPalpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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