Mouse anti-Human Siglec 5/Siglec 14 Monoclonal Antibody | anti-SIGLEC5/SIGLEC14 antibody

Siglec 5/Siglec 14 (Sialic Acid-binding Ig-like Lectin 5, CD170, CD33L2, OBBP2) (MaxLight 550)

Cat. Num. AAA6255290

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Siglec 5/Siglec 14; Monoclonal Antibody; Siglec 5/Siglec 14 (Sialic Acid-binding Ig-like Lectin 5; CD170; CD33L2; OBBP2) (MaxLight 550); anti-SIGLEC5/SIGLEC14 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1A5
• Specificity: Recognizes human Siglec-5/Siglec-14. Species Crossreactivity: chimpanzee
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.

• Applicable Applications for anti-SIGLEC5/SIGLEC14 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunoprecipitation (IP)
• Immunogen: Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
• Conjugate: MaxLight550
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14 followed by rabbit F(ab')2 anti mouse IgG:FITC.)

Product Categories/Family for anti-SIGLEC5/SIGLEC14 antibody

Antibodies; Lectins

References

1. Cornish, A.L. et al. (1998) Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33. Blood. 92: 2123-2132. 2. Avril, T. et al. (2005) Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine- based inhibitory motif phosphorylation. J. Biol. Chem. 280: 19843-19851. 3. Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution. P.N.A.S. 103: 7765- 7770. 4. Jaroenpool, J. et al. (2007) Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates. Cell Immunol. 250: 91-104. 5. Angata, T. et al. (2006) Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 20: 1964-1973.

NCBI and Uniprot Product Information

• NCBI GI #: 25009254
• NCBI GeneID: 8778
• UniProt Accession #: O15389; Q08ET2
• Molecular Weight: 60,715 Da
• NCBI Official Full Name: Sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2

NCBI Description

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]

Uniprot Description

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.