Human Regenerating islet-derived protein 3-alpha ELISA Kit | REG3A elisa kit

Human Regenerating islet-derived protein 3-alpha ELISA Kit

Cat. Num. AAA9426621

• Gene Names: REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
• Reactivity: Human
• Synonyms: Regenerating islet-derived protein 3-alpha; Human Regenerating islet-derived protein 3-alpha ELISA Kit; REG-3-alpha; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha; REG3A; HIP; PAP; REG3A elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human REG-3-alpha. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 0.78 ng/mL -50.0 ng/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for REG3A elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human regenerating islet-derived protein 3-alpha,REG-3-alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG-3-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for REG-3-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain REG-3-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of REG-3-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505605
• NCBI GeneID: 5068
• NCBI Accession #: NP_002571.1
• NCBI GenBank Nucleotide #: NM_002580.2
• UniProt Accession #: Q06141
• Molecular Weight: 19,395 Da
• NCBI Official Full Name: regenerating islet-derived protein 3-alpha
• NCBI Official Synonym Full Names: regenerating family member 3 alpha
• NCBI Official Symbol: REG3A
• NCBI Official Synonym Symbols: HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
• NCBI Protein Information: regenerating islet-derived protein 3-alpha
• UniProt Protein Name: Regenerating islet-derived protein 3-alpha
• Protein Family: Regenerating islet-derived protein
• UniProt Gene Name: REG3A
• UniProt Synonym Gene Names: HIP; PAP; PAP1; REG-3-alpha; HIP/PAP; Reg III-alpha

NCBI Description

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014]

Uniprot Description

REG3A: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: antimicrobial humoral response; cell proliferation; heterophilic cell adhesion; multicellular organismal development; negative regulation of keratinocyte differentiation