Western Blot (WB)
(Western Blot;Sample: Rat Liver lysate;Primary Ab: 2ug/ml Mouse Anti-Human SIGLEC8 Antibody;Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086032))
Mouse Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) Monoclonal Antibody | anti-SIGLEC8 antibody
Monoclonal Antibody to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)
Gene Names
SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
Reactivity
Homo sapiens (Human)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8); Monoclonal Antibody; Monoclonal Antibody to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8); CD & Adhesion molecule; Tumor immunity; Infection immunity; anti-SIGLEC8 antibody
Host
Mouse
Reactivity
Homo sapiens (Human)
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
D3
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SIGLEC8 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Source
Monoclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rat, Pig
Immunogen
Recombinant SIGLEC8 (Gly70~Ser336) expressed in E.coli (MBS2029916).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot;Sample: Rat Liver lysate;Primary Ab: 2ug/ml Mouse Anti-Human SIGLEC8 Antibody;Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086032))
Western Blot (WB)
(Western Blot;Sample: Rat Liver lysate;Primary Ab: 2ug/ml Mouse Anti-Human SIGLEC8 Antibody;Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086032))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue;Primary Ab: 30ug/ml Mouse Anti-Human SIGLEC8 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue;Primary Ab: 30ug/ml Mouse Anti-Human SIGLEC8 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sialic acid-binding Ig-like lectin 8 isoform 1
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 8
NCBI Official Symbol
SIGLEC8
NCBI Official Synonym Symbols
SAF2; SIGLEC-8; SIGLEC8L
NCBI Protein Information
sialic acid-binding Ig-like lectin 8
UniProt Protein Name
Sialic acid-binding Ig-like lectin 8
Protein Family
UniProt Gene Name
SIGLEC8
UniProt Synonym Gene Names
SAF2; Siglec-8; SAF-2
UniProt Entry Name
SIGL8_HUMAN
NCBI Description
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]
Research Articles on SIGLEC8
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Product Notes
The SIGLEC8 siglec8 (Catalog #AAA2111248) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the SIGLEC8 siglec8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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