Mouse SHPK Monoclonal Antibody | anti-SHPK antibody

SHPK antibody

Cat. Num. AAA834521

• Gene Names: SHPK; SHK; CARKL
• Applications: Western Blot
• Purity: SHPK antibody was purified by affinity chromatography.
• Synonyms: SHPK; Monoclonal Antibody; SHPK antibody; Monoclonal SHPK; Anti-SHPK; Sedoheptulokinase; SHK; CARKL; Carbohydrate kinase-like protein; Carbohydrate kinase-like; anti-SHPK antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1D2
• Purity/Purification: SHPK antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 478

• Applicable Applications for anti-SHPK antibody: Western Blot (WB)
• Application Notes: WB: 1:2000
• Biological Significance: SHPK has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation betweenthis novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changesin kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also richin Alu repetitive sequences, frequently involved in chromosomal rearrangements.
• Cross-Reactivity: Human
• Immunogen: SHPK antibody was raised in mouse using a full length recombinant protein of human SHPK (NP_037408) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SHPK protein (Right) or empty vector (Left) detected with SHPK antibody)

Related Product Information for anti-SHPK antibody

Mouse monoclonal SHPK antibody

Product Categories/Family for anti-SHPK antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 74315356
• NCBI GeneID: 23729
• NCBI Accession #: NP_037408
• NCBI GenBank Nucleotide #: NM_013276.2
• UniProt Accession #: Q9UHJ6; Q8WUH3; B2R640
• Molecular Weight: 51,491 Da
• NCBI Official Full Name: sedoheptulokinase
• NCBI Official Synonym Full Names: sedoheptulokinase
• NCBI Official Symbol: SHPK
• NCBI Official Synonym Symbols: SHK; CARKL
• NCBI Protein Information: sedoheptulokinase
• UniProt Protein Name: Sedoheptulokinase
• Protein Family: Sedoheptulokinase
• UniProt Gene Name: SHPK
• UniProt Synonym Gene Names: CARKL; SHK
• UniProt Entry Name: SHPK_HUMAN

NCBI Description

The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008]

Uniprot Description

SHPK: has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008]

Protein type: Kinase, other; EC 2.7.1.14

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: cytoplasm

Molecular Function: sedoheptulokinase activity; ATP binding

Biological Process: regulation of macrophage activation; regulation of inflammatory response; carbohydrate metabolic process; pentose-phosphate shunt, non-oxidative branch; phosphorylation