Mouse anti-E Coli Shiga toxin II B Monoclonal Antibody | anti-stx2B antibody

Anti-Shiga toxin II subunit B Antibody, Mouse Monoclonal

Cat. Num. AAA8110569

• Reactivity: E Coli
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: Shiga toxin II B; Monoclonal Antibody; Anti-Shiga toxin II subunit B Antibody; Mouse Monoclonal; Enterohemorrhagic E Coli (EHEC) stx2B/Shiga toxin II subunit B Antibody; Mouse MAb; anti-stx2B antibody

• Host: Mouse
• Reactivity: E Coli
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 13
• Specificity: Enterohemorrhagic E Coli (EHEC) STX2B
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-stx2B antibody: ELISA (EIA), ELISA (EIA) (Det)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Det): 1:1000-1:10,000; Antibody's applications have not been validated with corresponding E Coli.; ELISA (Det): This antibody will detect Enterohemorrhagic E). coli (EHEC) STX2B in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Immunogen: Recombinant Enterohemorrhagic E Coli (EHEC) STX2B protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Enterohemorrhagic E Coli (EHEC) STX2B. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-stx2B antibody

E Coli STX2B is a subunit of Stx2. Stx2, together with Stx1, formed a family of related toxins which are known as shiga toxins. Shiga toxins are mainly produced by the bacteria S. dysenteriae and the Shigatoxigenic group of Escherichia coli, which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E Coli (EHEC). A total of 3222 outbreak cases (including 39 deaths) have been reported in northern Germany in May through June 2011. The outbreak strain was typed as an enteroaggregative Shiga-toxin-producing E Coli O104:H4, producing extended-spectrum beta-lactamase. The toxin has two subunits-A and B. E Coli STX2B is the B subunit. It is a pentamer that binds to specific glycolipids on the host cell, specifically globotriaosylceramide. Following this, the A subunit is internalised and cleaved into two parts. Stx2 has been found to be approximately 400 times more toxic (as quantified by LD50 in mice) than Stx-1. The Stx1 and Stx2 B subunits form a pentameric structure that binds to globotriaosylceramide receptors on eukaryotic cells and promotes endocytosis.

References

Obata F. et al. (2008) Shiga Toxin 2 Affects the Central Nervous System through Receptor Globotriaosylceramide Localized to Neurons. J Infect Dis. 198 (9): 1398-406.Tironi-Farinati C. et al. (2010) Intracerebroventricular Shiga toxin 2 increases the expression of its receptor globotriaosylceramide and causes dendritic abnormalities. J Neuroimmunol. 222 (1-2): 48-61.Asakura H. et al. (2001) Phylogenetic diversity and similarity of active sites of Shiga toxin (stx) in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and animals. Epidemiol Infect. 127 (1): 27-36.

NCBI and Uniprot Product Information

• UniProt Accession #: Q93EY4