Mouse SHANK3 Monoclonal Antibody | anti-SHANK3 antibody

SHANK3 (SH3 and multiple ankyrin repeat domains 3, DEL22q13.3, KIAA1650, Proline-rich synapse-associated protein 2, prosap2, ProSAP2, PROSAP2, PSAP2, SH3 and multiple ankyrin repeat domains protein 3, Shank3)

Cat. Num. AAA606114

• Gene Names: SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; KIAA1650; DEL22q13.3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SHANK3; Monoclonal Antibody; SHANK3 (SH3 and multiple ankyrin repeat domains 3; DEL22q13.3; KIAA1650; Proline-rich synapse-associated protein 2; prosap2; ProSAP2; PROSAP2; PSAP2; SH3 and multiple ankyrin repeat domains protein 3; Shank3); Anti -SHANK3 (SH3 and multiple ankyrin repeat domains 3; anti-SHANK3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 10F768
• Specificity: Recognizes at Shank3. Species Crossreactivity: human. No cross-reactivity against Shank1 or Shank2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.

• Applicable Applications for anti-SHANK3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot:1-10ug/ml; Immunohistochemistry:0.1-1ug/ml
• Immunogen: Synthetic peptide aa 840-857 PEKLPGSLRKGIPRTKSV) of rat Shank3 (also known as SH3 and multiple ankyrin repeat domains protein 3, prolinerich synapse- associated protein 2, ProSAP2 and SPANK- 2, accession number Q9JLU4).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analyiss of SHANK3 in 15ug rat brain membrane lysate: Membrane was blocked with 1.5% BSA for 30 mins. at RT followed by incubation with MBS606114 at 1:1000 dilution for 2 hrs at RT. HRP-conjugated sheep anti-mouse IgG was used as secondary antibody.)

Immunocytochemistry (ICC)

(IC/IC analysis of SHANK3 in HaCaT cells. Cells were fixed with 100% methanol for 10 mins. at -20°C. Incubation with MBS606114 at 1:100 dilution was carried out for 1 hour at RT. Incubation with FITC-conjugated goat anti-mouse IgG secondary antibody (green) was performed at 1 hr at RT.|Antigen localization: Borderline positive.)

Immunohistochemistry (IHC)

(IHC analysis of SHANK3 in mouse backskin tissue: Tissue was fixed with Bouin’s fixative and paraffin-embedded. Incubation with MBS606114 at 1:100 dilution was carried out for 1 hour at RT. Incubation with FITC-conjugated goat anti-mouse IgG secondary antibody (green) was performed at 1 hr at RT.|Antigen localization: Early stages of fillagrin-like and dermal staining.)

Related Product Information for anti-SHANK3 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or

Product Categories/Family for anti-SHANK3 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 122937241
• NCBI GeneID: 85358
• NCBI Accession #: NP_001073889.1
• NCBI GenBank Nucleotide #: NM_001080420.1
• UniProt Accession #: Q3S3B4; Q6P5A2; Q9BYB0
• Molecular Weight: 6,339 Da
• NCBI Official Full Name: SH3 and multiple ankyrin repeat domains protein 3
• NCBI Official Synonym Full Names: SH3 and multiple ankyrin repeat domains 3
• NCBI Official Symbol: SHANK3
• NCBI Official Synonym Symbols: PSAP2; SCZD15; PROSAP2; SPANK-2; KIAA1650; DEL22q13.3
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 3; shank postsynaptic density protein; proline rich synapse associated protein 2; proline-rich synapse-associated protein 2
• UniProt Protein Name: SHANK3
• Protein Family: SH3 and multiple ankyrin repeat domains protein
• UniProt Gene Name: SHANK3
• UniProt Entry Name: Q3S3B4_HUMAN

NCBI Description

This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD) which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene are a major causitive factor in the neurological symptoms of 22q13.3 deletion syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. [provided by RefSeq]

Uniprot Description

SHANK3: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B. Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome). Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15). SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.3

Cellular Component: postsynaptic membrane; neuron projection; cytoplasm; plasma membrane; dendritic spine; cell junction

Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; identical protein binding; protein binding; zinc ion binding; GKAP/Homer scaffold activity; actin binding; SH3 domain binding

Biological Process: positive regulation of synapse structural plasticity; negative regulation of actin filament bundle formation; synaptogenesis; striatal medium spiny neuron differentiation; adult behavior; MAPKKK cascade; brain morphogenesis; vocal learning; social behavior; learning; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell volume; positive regulation of long-term neuronal synaptic plasticity; memory

Disease: Schizophrenia 15; Phelan-mcdermid Syndrome