Mouse anti-Mouse, Rat SHANK1/SHANK3 Monoclonal Antibody | anti-Shank3 antibody

SHANK1/SHANK3 Antibody: ATTO 488

Cat. Num. AAA804927

• Gene Names: Shank3; Prosap2
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Synonyms: SHANK1/SHANK3; Monoclonal Antibody; SHANK1/SHANK3 Antibody: ATTO 488; Shank postsynaptic density protein; SH3 and multiple ankyrin repeat domains 3; Proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; PSAP2;; SH3 and multiple ankyrin repeat domains protein 3; anti-Shank3 antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: S367-51
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1740

• Applicable Applications for anti-Shank3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: 1:1000 WB
• Conjugate: ATTO 488
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.)

Related Product Information for anti-Shank3 antibody

Background Info: Detects a 190 kDa protein. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

Scientific Background: SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.

Product Categories/Family for anti-Shank3 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 11067399
• NCBI GeneID: 59312
• NCBI Accession #: NP_067708.1
• NCBI GenBank Nucleotide #: NM_021676.1
• UniProt Accession #: Q9JLU4; Q9WUY7; Q9WV47
• Molecular Weight: 122,694 Da
• NCBI Official Full Name: SH3 and multiple ankyrin repeat domains protein 3
• NCBI Official Synonym Full Names: SH3 and multiple ankyrin repeat domains 3
• NCBI Official Symbol: Shank3
• NCBI Official Synonym Symbols: Prosap2
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 3; SPANK-2; SH3/ankyrin domain gene 3; Shank postsynaptic density protein 3a; proline rich synapse associated protein 2; proline-rich synapse-associated protein 2
• UniProt Protein Name: SH3 and multiple ankyrin repeat domains protein 3
• Protein Family: SH3 and multiple ankyrin repeat domains protein
• UniProt Gene Name: Shank3
• UniProt Synonym Gene Names: Prosap2; Shank3; ProSAP2
• UniProt Entry Name: SHAN3_RAT

NCBI Description

may be involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

Uniprot Description

SHANK3: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B. Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome). Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15). SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: postsynaptic membrane; neuron projection; cell; postsynaptic density; cytoplasm; dendritic spine; plasma membrane; synapse; intracellular; cell junction

Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; identical protein binding; protein binding; protein self-association; zinc ion binding; GKAP/Homer scaffold activity; actin binding; SH3 domain binding

Biological Process: negative regulation of actin filament bundle formation; MAPKKK cascade; learning; vocal learning; social behavior; positive regulation of synaptic transmission, glutamatergic; positive regulation of long-term neuronal synaptic plasticity; protein oligomerization; memory; positive regulation of synapse structural plasticity; synaptogenesis; striatal medium spiny neuron differentiation; adult behavior; embryonic epithelial tube formation; brain morphogenesis; neuromuscular process controlling balance; negative regulation of cell volume