Mouse SH2B3 Monoclonal Antibody | anti-SH2B3 antibody

SH2B3 antibody

Cat. Num. AAA833253

• Gene Names: SH2B3; LNK; IDDM20
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: SH2B3 antibody was purified by affinity chromatography.
• Synonyms: SH2B3; Monoclonal Antibody; SH2B3 antibody; Monoclonal SH2B3; Anti-SH2B3; SH2B adaptor protein 3; LNK; IDDM20; Lymphocyte-specific adapter protein Lnk; Signal transduction protein Lnk; SHB3 2; SHB3-2; anti-SH2B3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1F6
• Purity/Purification: SH2B3 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 575

• Applicable Applications for anti-SH2B3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:100; FC: 1:100; WB: 1:500
• Biological Significance: SH2B3 a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokinesignaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibilityto celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus.
• Cross-Reactivity: Human
• Immunogen: SH2B3 antibody was raised in mouse using a full length recombinant protein of human SH2B3 (NP_005466) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant SH2B3 protein (red) or empty vector (blue) stained using SH2B3 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SH2B3 protein (Right) or empty vector (Left) detected with SH2B3 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SH2B3 protein using SH2B3 antibody)

Related Product Information for anti-SH2B3 antibody

Mouse monoclonal SH2B3 antibody

Product Categories/Family for anti-SH2B3 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4885455
• NCBI GeneID: 10019
• NCBI Accession #: NP_005466
• NCBI GenBank Nucleotide #: NM_005475.2
• UniProt Accession #: Q9UQQ2; O95184; B9EGG5
• Molecular Weight: 63,225 Da
• NCBI Official Full Name: SH2B adapter protein 3 isoform 1
• NCBI Official Synonym Full Names: SH2B adaptor protein 3
• NCBI Official Symbol: SH2B3
• NCBI Official Synonym Symbols: LNK; IDDM20
• NCBI Protein Information: SH2B adapter protein 3
• UniProt Protein Name: SH2B adapter protein 3
• Protein Family: SH2B adapter protein
• UniProt Gene Name: SH2B3
• UniProt Synonym Gene Names: LNK
• UniProt Entry Name: SH2B3_HUMAN

NCBI Description

This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

LNK: Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase. Genetic variations in SH2B3 are associated with susceptibility to celiac disease type 13 (CELIAC13)[MIM:612011]; also known as susceptibility to gluten-sensitive enteropathy type 13. Celiac disease is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. Genetic variations in SH2B3 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM); also known as diabetes mellitus type 1. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis. Belongs to the SH2B adapter family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: cytosol

Molecular Function: signal transducer activity; protein binding; protein complex binding; phosphate binding

Biological Process: embryonic hemopoiesis; cell differentiation; blood coagulation

Disease: Myelofibrosis; Erythrocytosis, Familial, 1; Thrombocythemia 1