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Western Blot (WB) (Western Blot; Sample: Lane1: Human Serum; Lane2: Human Placenta lysate; Primary Ab: 3ug/ml Mouse Anti-Human SHBG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Mouse anti-Human Sex Hormone Binding Globulin (SHBG) Monoclonal Antibody | anti-SHBG antibody

Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG)

Gene Names
SHBG; ABP; SBP; TEBG
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G Affinity Chromatography
Synonyms
Sex Hormone Binding Globulin (SHBG); Monoclonal Antibody; Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG); ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin; anti-SHBG antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C7
Specificity
The antibody is a mouse monoclonal antibody raised against SHBG. It has been selected for its ability to recognize SHBG in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G Affinity Chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Pro51~Asp218
Applicable Applications for anti-SHBG antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
Immunogen Sequence
Pro51~Asp218
Cross Reactivity
Human
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Lane1: Human Serum; Lane2: Human Placenta lysate; Primary Ab: 3ug/ml Mouse Anti-Human SHBG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot; Sample: Lane1: Human Serum; Lane2: Human Placenta lysate; Primary Ab: 3ug/ml Mouse Anti-Human SHBG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10ug/ml Mouse Anti-Human SHBG Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10ug/ml Mouse Anti-Human SHBG Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Product Categories/Family for anti-SHBG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,779 Da
NCBI Official Full Name
sex hormone-binding globulin isoform 1
NCBI Official Synonym Full Names
sex hormone-binding globulin
NCBI Official Symbol
SHBG
NCBI Official Synonym Symbols
ABP; SBP; TEBG
NCBI Protein Information
sex hormone-binding globulin; sex steroid-binding protein; testosterone-binding beta-globulin; testosterone-estrogen-binding globulin; testosterone-estradiol-binding globulin; testis-specific androgen-binding protein
UniProt Protein Name
Sex hormone-binding globulin
UniProt Gene Name
SHBG
UniProt Synonym Gene Names
SHBG; SBP; ABP; TeBG
UniProt Entry Name
SHBG_HUMAN

NCBI Description

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein binds each steroid molecule as a dimer formed from identical or nearly identical monomers. The use of alternate promoters and alternatively spliced transcripts have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: extracellular region

Molecular Function: androgen binding

Biological Process: primary spermatocyte growth

Research Articles on SHBG

Similar Products

Product Notes

The SHBG shbg (Catalog #AAA2111544) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Sex Hormone Binding Globulin (SHBG) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sex Hormone Binding Globulin (SHBG) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the SHBG shbg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Pro51~Asp2 18. It is sometimes possible for the material contained within the vial of "Sex Hormone Binding Globulin (SHBG), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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