Mouse anti-Human Secretory Leukocyte Protease Inhibitor Monoclonal Antibody | anti-SLPI antibody

Secretory Leukocyte Protease Inhibitor (SLPI) (Biotin)

Cat. Num. AAA601321

• Gene Names: SLPI; ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I; SLPI
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified IgG
• Synonyms: Secretory Leukocyte Protease Inhibitor; Monoclonal Antibody; Secretory Leukocyte Protease Inhibitor (SLPI) (Biotin); Anti -Secretory Leukocyte Protease Inhibitor (SLPI) (Biotin); anti-SLPI antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6A617
• Specificity: Reacts specificly with secretory leukocyte proteinase inhibitor (SLPI; also known as antileukoprotease or ALP).
• Purity/Purification: Affinity Purified; Purified IgG
• Form/Format: Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA. Conjugated to Biotin.

• Applicable Applications for anti-SLPI antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Can be used for Immunohistology on frozen and paraffin sections. Furthermore the antibody is useful for Western Blot and immunoassays.; Dilution: Immunohistology: 1:10; Western Blot: 1:10
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLPI antibody

SLPI is a 11.7kD cationic inhibitor of neutrophil elastase and to a lesser extent of cathepsin G. It is locally produced by epithelial cells in the lung, skin and other organs and by Polymorphonuclear leukocytes (PMN) and (in mice) by macrophages. In addition to its proteinase inhibitory properties that may serve to protect against proteolytic injury, it was recently shown that SLPI also displays several other functions such as antimicrobial and anti-inflammatory activities. These appear to be independent of its ability to inhibit PMN serine proteinases. SLPI has also been demonstrated to display antibacterial and antifungal activity at concentrations in which SLPI is present in mucosal secretions including those of the lung. Another possible role for SLPI is inhibition of protein-disulfide isomerase that is considered essential for invasion of a cell by the Human Immunodeficiency Virus (HIV).

Product Categories/Family for anti-SLPI antibody

Antibodies; Abs to Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 758101
• NCBI GeneID: 6590
• UniProt Accession #: P03973; P07757; B2R5H8; Q6LDI0
• Molecular Weight: 14,326 Da
• NCBI Official Full Name: secretory leukocyte protease inhibitor (SLPI)
• NCBI Official Synonym Full Names: secretory leukocyte peptidase inhibitor
• NCBI Official Symbol: SLPI
• NCBI Official Synonym Symbols: ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I; SLPI
• NCBI Protein Information: antileukoproteinase; HUSI-1; protease inhibitor WAP4; mucus proteinase inhibitor; seminal proteinase inhibitor; WAP four-disulfide core domain 4; WAP four-disulfide core domain protein 4; secretory leukocyte protease inhibitor (antileukoproteinase)
• UniProt Protein Name: Antileukoproteinase
• Protein Family: Antileukoproteinase
• UniProt Gene Name: SLPI
• UniProt Synonym Gene Names: WAP4; WFDC4
• UniProt Entry Name: SLPI_HUMAN

NCBI Description

This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity. [provided by RefSeq]

Uniprot Description

SLPI: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: extracellular matrix

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; endopeptidase inhibitor activity

Biological Process: negative regulation of viral genome replication; negative regulation of protein binding