Testing Data
Mouse anti-Human SECIS Binding Protein 2 Monoclonal Antibody | anti-SECISBP2 antibody
SECIS Binding Protein 2 (SECISBP2, DKFZp686C09169, SBP2)
Gene Names
SECISBP2; SBP2; DKFZp686C09169; SECISBP2
Reactivity
Human
Applications
Western Blot, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
SECIS Binding Protein 2; Monoclonal Antibody; SECIS Binding Protein 2 (SECISBP2; DKFZp686C09169; SBP2); Anti -SECIS Binding Protein 2 (SECISBP2; anti-SECISBP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8J142
Specificity
Recognizes human SECIS Binding Protein 2.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 1% BSA and 0.05% sodium azide.
Applicable Applications for anti-SECISBP2 antibody
Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Dot Blot and Western Blot.
Immunogen
Partial sequence of recombinant full-length protein to human SECIS Binding Protein 2
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Testing Data
Testing Data
Product Categories/Family for anti-SECISBP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
95,462 Da
NCBI Official Full Name
SECIS binding protein 2
NCBI Official Synonym Full Names
SECIS binding protein 2
NCBI Official Symbol
SECISBP2
NCBI Official Synonym Symbols
SBP2; DKFZp686C09169; SECISBP2
NCBI Protein Information
selenocysteine insertion sequence-binding protein 2; SECIS-binding protein 2; selenocysteine insertion sequence binding protein 2
UniProt Protein Name
Selenocysteine insertion sequence-binding protein 2
Protein Family
UniProt Gene Name
SECISBP2
UniProt Synonym Gene Names
SBP2
UniProt Entry Name
SEBP2_HUMAN
NCBI Description
The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. [provided by RefSeq]
Uniprot Description
SECISBP2: Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM). This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation
Chromosomal Location of Human Ortholog: 9q22.2
Cellular Component: nucleoplasm; mitochondrion; ribonucleoprotein complex
Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding
Biological Process: selenocysteine incorporation
Disease: Thyroid Hormone Metabolism, Abnormal
Research Articles on SECISBP2
1. Observational study of gene-disease association. (HuGE Navigator)
2. Results demonstrate that SECIS-binding protein 2 is required for protection against reactive oxygen species-induced cellular damage and cell survival.
3. SBP2 gene mutation producing early arrest in synthesis of full-length molecule. Demonstration that SBP2 isoforms containing all functional domains could be synthesized from three downstream ATGs explains the relatively mild phenotype caused by this defect
4. ca. 70% of the SBP2 sequence is disordered, whereas the RNA binding domain appears to be folded and functional.
5. Seleniuim is obviously not a limiting factor in the SBP2 deficient individuals when regular daily selenium intake is provided
6. CUG-BP1 and HuR act as factors that bind to the SBP2 3' UTR, which suggests that TTR-RBPs play a role in the regulation of SBP2
7. SECIS binding induces a conformational change in SBP2 that recruits eEFSec, which in concert with the Sec incorporation domain gains access to the ribosomal A site
8. A report of a complex splicing pattern in the 5'-region of human SECISBP2, wherein at least eight splice variants encode five isoforms with varying N-terminal sequence.
9. Data suggest that SBP2 is a major determinant in dictating the hierarchy of selenoprotein synthesis via differential selenoprotein mRNA translation and sensitivity to nonsense-mediated decay.
10. Oxidative stress induces nuclear accumulation of SBP2 via oxidation of cysteine residues within a redox-sensitive cysteine-rich domain.
2. Results demonstrate that SECIS-binding protein 2 is required for protection against reactive oxygen species-induced cellular damage and cell survival.
3. SBP2 gene mutation producing early arrest in synthesis of full-length molecule. Demonstration that SBP2 isoforms containing all functional domains could be synthesized from three downstream ATGs explains the relatively mild phenotype caused by this defect
4. ca. 70% of the SBP2 sequence is disordered, whereas the RNA binding domain appears to be folded and functional.
5. Seleniuim is obviously not a limiting factor in the SBP2 deficient individuals when regular daily selenium intake is provided
6. CUG-BP1 and HuR act as factors that bind to the SBP2 3' UTR, which suggests that TTR-RBPs play a role in the regulation of SBP2
7. SECIS binding induces a conformational change in SBP2 that recruits eEFSec, which in concert with the Sec incorporation domain gains access to the ribosomal A site
8. A report of a complex splicing pattern in the 5'-region of human SECISBP2, wherein at least eight splice variants encode five isoforms with varying N-terminal sequence.
9. Data suggest that SBP2 is a major determinant in dictating the hierarchy of selenoprotein synthesis via differential selenoprotein mRNA translation and sensitivity to nonsense-mediated decay.
10. Oxidative stress induces nuclear accumulation of SBP2 via oxidation of cysteine residues within a redox-sensitive cysteine-rich domain.
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Product Notes
The SECISBP2 secisbp2 (Catalog #AAA606332) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SECIS Binding Protein 2 (SECISBP2, DKFZp686C09169, SBP2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SECIS Binding Protein 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in Dot Blot and Western Blot. Researchers should empirically determine the suitability of the SECISBP2 secisbp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SECIS Binding Protein 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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