Testing Data
(Detection of endogenous scramblase 1|Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MBS601869 (0.5ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec).|lane 1: HeLa; lane 2: HepG2; lane 3: HEK-293 ; lane 4: SY5Y; lane 5: Jurkat; lane 6: U937; lane 7: K562; lane 8: THP)
Mouse anti-Human Scramblase 1 Monoclonal Antibody | anti-scrm-1 antibody
Scramblase 1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by subsequent thiophilic absorption and size exclusion chromatography.
Synonyms
Scramblase 1; Monoclonal Antibody; Anti -Scramblase 1; anti-scrm-1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D755
Specificity
Recognizes the N-terminus of human Scramblase 1. Detects a band of ~35kD by Western Blot.
Purity/Purification
Purified
Purified by subsequent thiophilic absorption and size exclusion chromatography.
Purified by subsequent thiophilic absorption and size exclusion chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide, PEG, sucrose.
Applicable Applications for anti-scrm-1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween-20 based blocking and blot incubation buffer.
Optimal dilution to be determined by researcher.
Dilution: Western Blot: 1ug/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween-20 based blocking and blot incubation buffer.
Optimal dilution to be determined by researcher.
Immunogen
Peptide corresponding to a portion of N-terminal human Scramblase 1 conjugated to hemocyanin.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Detection of endogenous scramblase 1|Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MBS601869 (0.5ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec).|lane 1: HeLa; lane 2: HepG2; lane 3: HEK-293 ; lane 4: SY5Y; lane 5: Jurkat; lane 6: U937; lane 7: K562; lane 8: THP)
Testing Data
(Detection of endogenous scramblase 1|Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MBS601869 (0.5ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec).|lane 1: HeLa; lane 2: HepG2; lane 3: HEK-293 ; lane 4: SY5Y; lane 5: Jurkat; lane 6: U937; lane 7: K562; lane 8: THP)
Related Product Information for anti-scrm-1 antibody
Scramblase 1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor - EGF). Depending on its palmitoylation state, Scramblase 1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase 1 is a substrate for src kinases.
Product Categories/Family for anti-scrm-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,084 Da
NCBI Official Full Name
SCRaMblase (phospholipid scramblase) family member (scrm-1)
NCBI Official Symbol
scrm-1
NCBI Protein Information
SCRaMblase (phospholipid scramblase) family member (scrm-1)
UniProt Protein Name
Protein T22H2.5a, confirmed by transcript evidence
UniProt Gene Name
scrm-1
UniProt Entry Name
Q7JKP1_CAEEL
Similar Products
Product Notes
The scrm-1 scrm-1 (Catalog #AAA601869) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Scramblase 1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Scramblase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween-20 based blocking and blot incubation buffer. Optimal dilution to be determined by researcher. Researchers should empirically determine the suitability of the scrm-1 scrm-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Scramblase 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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