Western Blot (WB)
(Fig. Western blot analysis of Arabidopsis with Rubisco (Large Chain) monoclonal antibody at 1:2000 (lane A) and 1:5000 (lane B) dilutions, seperately.)
Mouse anti-Plants Rubisco (Large Chain) Monoclonal Antibody | anti-rbcL antibody
Anti-Rubisco (Large Chain) Monoclonal Antibody (9Y6)
Reactivity
Plants
Applications
Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Synonyms
Rubisco (Large Chain); Monoclonal Antibody; Anti-Rubisco (Large Chain) Monoclonal Antibody (9Y6); Rubisco; RuBisCO large subunit; Ribulose bisphosphate carboxylase large chain; anti-rbcL antibody
Host
Mouse
Reactivity
Plants
Clonality
Monoclonal
Isotype
Mouse IgG
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Applicable Applications for anti-rbcL antibody
Western Blot (WB)
Application Notes
WB: 1:2000-1:5000
Tag
Rubisco
Observed Band (KD)
53
Immunogen
Recombinant Protein
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Western Blot (WB)
(Fig. Western blot analysis of Arabidopsis with Rubisco (Large Chain) monoclonal antibody at 1:2000 (lane A) and 1:5000 (lane B) dilutions, seperately.)
Western Blot (WB)
(Fig. Western blot analysis of Arabidopsis with Rubisco (Large Chain) monoclonal antibody at 1:2000 (lane A) and 1:5000 (lane B) dilutions, seperately.)
Related Product Information for anti-rbcL antibody
Description: Featured Anti-Rubisco (Large Chain) Monoclonal Antibody (9Y6), specially designed for your immunoassay as internal control for plants.
Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55kDa) is coded by the chloroplast rbcL gene, and the small subunit (15kDa) is coded by a family of nuclear rbcS genes.
Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55kDa) is coded by the chloroplast rbcL gene, and the small subunit (15kDa) is coded by a family of nuclear rbcS genes.
Product Categories/Family for anti-rbcL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,955 Da
NCBI Official Full Name
ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast)
NCBI Official Symbol
rbcL
NCBI Protein Information
RuBisCO large subunit
UniProt Protein Name
Ribulose bisphosphate carboxylase large chain
Protein Family
UniProt Gene Name
rbcL
UniProt Synonym Gene Names
RuBisCO large subunit
Uniprot Description
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
Research Articles on rbcL
Similar Products
Product Notes
The rbcL rbcl (Catalog #AAA9719119) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rubisco (Large Chain) Monoclonal Antibody (9Y6) reacts with Plants and may cross-react with other species as described in the data sheet. AAA Biotech's Rubisco (Large Chain) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2000-1:5000. Researchers should empirically determine the suitability of the rbcL rbcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rubisco (Large Chain), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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