ITPR3 Polyclonal Antibody | anti-ITPR3 antibody

Anti-ITPR3 Antibody

Cat. Num. AAA177776

• Gene Names: ITPR3; IP3R; IP3R3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: ITPR3; Polyclonal Antibody; Anti-ITPR3 Antibody; Inositol 1; 4; 5-trisphosphate receptor type 3; 5-trisphosphate receptor; FLJ36205; 5 trisphosphate receptor type 3; InsP3R3; IP3 receptor; IP3 receptor isoform 3; IP3R 3; IP3R; IP3R3; ITPR 3; ITPR3_HUMAN; Type 3 inositol 1; 5 trisphosphate receptor; Type 3 InsP3 receptor; inositol 1; type 3; anti-ITPR3 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 2671

• Applicable Applications for anti-ITPR3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ITPR3 (70-95aa QYWKAKQTKQDKEKIADVVLLQKLQH), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- ITPR3 Picoband antibody, MBS177776, Western blottingAll lanes: Anti ITPR3 (MBS177776) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugPredicted bind size: 304KDObserved bind size: 304KD )

Immunohistochemistry (IHC)

(Anti- ITPR3 Picoband antibody, MBS177776, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- ITPR3 Picoband antibody, MBS177776, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- ITPR3 Picoband antibody, MBS177776, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-ITPR3 antibody

Description: Rabbit IgG polyclonal antibody for Inositol 1,4,5-trisphosphate receptor type 3(ITPR3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: ITPR3 encodes a receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. The receptor contains a calcium channel at the C-terminus and the ligand-binding site at the N-terminus. Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth. ITP3 channels serve an important role in the taste transduction pathway of sweet, bitter and umami tastes the gustatory system. ITP3 channels allow the flow of Calcium out of the endoplasmic reticulum in response to IP3. Calcium cations result in the activation of TRPM5 which leads to a depolarisation generating potential and an action potential.

References

1. "Entrez Gene: inositol 1". 2. Chaudhari N, Roper SD (August 2010)."The cell biology of taste". J. Cell Biol.190 (3): 285-96. 3. Yamamoto-Hino M, Sugiyama T, Hikichi K et al. (1994). "Cloning and characterization of human type 2 and type 3 inositol 1,4,5-trisphosphate receptors".Recept. Channels 2 (1): 9-22.

NCBI and Uniprot Product Information

• NCBI GI #: 153945846
• NCBI GeneID: 3710
• NCBI Accession #: NP_002215.2
• NCBI GenBank Nucleotide #: NM_002224.3
• UniProt Accession #: Q14573; Q14649; Q5TAQ2
• Molecular Weight: 304,106 Da
• NCBI Official Full Name: inositol 1,4,5-trisphosphate receptor type 3
• NCBI Official Synonym Full Names: inositol 1,4,5-trisphosphate receptor type 3
• NCBI Official Symbol: ITPR3
• NCBI Official Synonym Symbols: IP3R; IP3R3
• NCBI Protein Information: inositol 1,4,5-trisphosphate receptor type 3
• UniProt Protein Name: Inositol 1,4,5-trisphosphate receptor type 3
• Protein Family: Inositol 1,4,5-trisphosphate receptor
• UniProt Gene Name: ITPR3
• UniProt Synonym Gene Names: IP3R 3; InsP3R3; Type 3 InsP3 receptor
• UniProt Entry Name: ITPR3_HUMAN

NCBI Description

This gene encodes a receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. The receptor contains a calcium channel at the C-terminus and the ligand-binding site at the N-terminus. Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth. [provided by RefSeq, Aug 2010]

Uniprot Description

IP3R3: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Expressed in intestinal crypt and villus epithelial cells. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylated on tyrosine residues. Phosphorylated by AKT1 on serine and/or threonine residues. Belongs to the InsP3 receptor family.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Channel, calcium

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: apical part of cell; brush border; cell soma; cytoplasm; cytosol; dendrite; endoplasmic reticulum; endoplasmic reticulum membrane; integral to plasma membrane; membrane; myelin sheath; nuclear outer membrane; nucleolus; nucleoplasm; perinuclear region of cytoplasm; plasma membrane; receptor complex

Molecular Function: calcium ion binding; calcium-release channel activity; inositol 1,3,4,5 tetrakisphosphate binding; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity; inositol hexakisphosphate binding; phosphoinositide binding; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; inositol phosphate-mediated signaling; memory; platelet activation; protein heterooligomerization; protein homooligomerization; regulation of insulin secretion; release of sequestered calcium ion into cytosol; response to calcium ion; sensory perception of bitter taste; sensory perception of sweet taste; sensory perception of umami taste

Disease: Diabetes Mellitus, Insulin-dependent