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Western Blot (WB)
(Western blot analysis of RSK2 on human RSK2 recombinant protein using anti-RSK2 antibody at 1/1, 000 dilution.)
Mouse anti-Human RSK2 Monoclonal Antibody | anti-RSK2 antibody
RSK2 Antibody
Gene Names
RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
RSK2; Monoclonal Antibody; RSK2 Antibody; 90 kDa ribosomal protein S6 kinase 3 antibody; CLS antibody; HU 3 antibody; HU2 antibody; HU3 antibody; Insulin stimulated protein kinase 1 antibody; Insulin-stimulated protein kinase 1 antibody; ISPK-1 antibody; ISPK1 antibody; KS6A3_HUMAN antibody; MAP kinase activated protein kinase 1b antibody; MAP kinase-activated protein kinase 1b antibody; MAPK activated protein kinase 1b antibody; MAPK-activated protein kinase 1b antibody; MAPKAP kinase 1b antibody; MAPKAPK 1b antibody; MAPKAPK-1b antibody; MAPKAPK1B antibody; Mental retardation; X linked 19 antibody; MRX19 antibody; OTTHUMP00000023036 antibody; p90 RSK2 antibody; p90 RSK3 antibody; p90-RSK 3 antibody; p90RSK3 antibody; pp90RSK2 antibody; Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody; Ribosomal protein S6 kinase alpha 3 antibody; Ribosomal protein S6 kinase alpha-3 antibody; Ribosomal protein s6 kinase ii alpha 2 antibody; Ribosomal S6 kinase 2 antibody; Rps6ka3 antibody; RSK antibody; RSK-2 antibody; RSK2 antibody; S6 kinase 2 antibody; S6K alpha3 antibody; S6K-alpha-3 antibody; anti-RSK2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
D2-E10
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RSK2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of RSK2 on human RSK2 recombinant protein using anti-RSK2 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of RSK2 on human RSK2 recombinant protein using anti-RSK2 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of RSK2 on different cell lysates using anti-RSK2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2)
Western Blot (WB)
(Western blot analysis of RSK2 on different cell lysates using anti-RSK2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2)
Immunocytochemistry (ICC)
(ICC staining RSK2 (green) and actin filaments (red) in epG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RSK2 (green) and actin filaments (red) in epG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells with RSK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells with RSK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Related Product Information for anti-RSK2 antibody
The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase b, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains.
Product Categories/Family for anti-RSK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
740
NCBI Official Full Name
ribosomal protein S6 kinase alpha-3
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 3
NCBI Official Symbol
RPS6KA3
NCBI Official Synonym Symbols
CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
NCBI Protein Information
ribosomal protein S6 kinase alpha-3; RSK-2; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; MAPK-activated protein kinase 1b; insulin-stimulated protein kinase 1; MAP kinase-activated protein kinase 1b
UniProt Protein Name
Ribosomal protein S6 kinase alpha-3
UniProt Gene Name
RPS6KA3
UniProt Synonym Gene Names
ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
UniProt Entry Name
KS6A3_HUMAN
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Product Notes
The RSK2 rps6ka3 (Catalog #AAA9435312) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RSK2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RSK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 ICC: 1:50-1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the RSK2 rps6ka3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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