Mouse RPS6KA5/MSK1 Monoclonal Antibody | anti-RPS6KA5 antibody

RPS6KA5/MSK1 Mouse Monoclonal Antibody [Clone PCRP-RPS6KA5-1A8]

Cat. Num. AAA4382372

• Gene Names: RPS6KA5; MSK1; RLPK; MSPK1
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: RPS6KA5/MSK1; Monoclonal Antibody; RPS6KA5/MSK1 Mouse Monoclonal Antibody [Clone PCRP-RPS6KA5-1A8]; 90kDa ribosomal protein S6 kinase 5; EC 2. 7. 11. 1; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSKL; S6K alpha 5; anti-RPS6KA5 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-RPS6KA5-1A8
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-RPS6KA5 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; WB: 1-2ug/ml
• Immunogen: Recombinant full-length human RPS6KA5 protein
• Cellular Localization: Nucleus, Cytoplasm, Predominantly nuclear, Partially cytoplasmic
• Positive Control: HeLa or JAR cells. Ubiquitous nuclear expression. Human prostate, tonsil or brain.
• Hu-chromosome Location: 14q32.11
• Unigene: 510225
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. RPS6KA5/MSK1 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red). )

Related Product Information for anti-RPS6KA5 antibody

The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.

NCBI and Uniprot Product Information

• NCBI GI #: 32528295
• NCBI GeneID: 9252
• NCBI Accession #: NP_004746.2
• NCBI GenBank Nucleotide #: NM_004755.2
• UniProt Accession #: O75582; O95316; Q96AF7
• Molecular Weight: 89,865 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-5 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 5
• NCBI Official Symbol: RPS6KA5
• NCBI Official Synonym Symbols: MSK1; RLPK; MSPK1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-5; RSKL; S6K-alpha-5; RSK-like protein kinase; 90 kDa ribosomal protein S6 kinase 5; nuclear mitogen- and stress-activated protein kinase 1
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-5
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA5
• UniProt Synonym Gene Names: MSK1; S6K-alpha-5; RSKL
• UniProt Entry Name: KS6A5_HUMAN

Uniprot Description

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; histone phosphorylation; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; inflammatory response; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; activation of CREB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of cytokine production; toll-like receptor signaling pathway; innate immune response; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent