Mouse anti-Human, Mouse RPA3 Monoclonal Antibody | anti-RPA3 antibody

Mouse Monoclonal [clone 111] (IgG1) to Human RPA3

Cat. Num. AAA245085

• Gene Names: RPA3; REPA3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: RPA3; Monoclonal Antibody; Mouse Monoclonal [clone 111] (IgG1) to Human RPA3; REPA3; Replication factor A protein 3; Replication protein A3 (14kD); Replication protein A3; 14kDa; RF-A protein 3; RP-A p14; RPA14; anti-RPA3 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 111
• Specificity: Full-length human RPA-14 expressed in E Coli.
• Purity/Purification: Protein G Purified
• Form/Format: PBS
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 121

• Applicable Applications for anti-RPA3 antibody: Immunohistochemistry (IHC), Immunohistochemistry Paraffin (IHC-P), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-P: 5 µg/ml; IHC: MBS245085 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245085 was determined to be 5 ug/ml.; ; (Optimal dilution to be determined by the researcher)
• Antigen Type: Recombinant protein
• Immunogen: RPA3 antibody was raised against full-length human RPA-14 expressed in E. coli.
• Immunogen Species: RPA3 antibody was raised againist Human.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20°C; Short term: 4°C.; Avoid repeat freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 4506587
• NCBI GeneID: 6119
• NCBI Accession #: NP_002938.1
• NCBI GenBank Nucleotide #: NM_002947.3
• UniProt Accession #: P35244; Q549U6
• NCBI Official Full Name: replication protein A 14 kDa subunit
• NCBI Official Synonym Full Names: replication protein A3, 14kDa
• NCBI Official Symbol: RPA3
• NCBI Official Synonym Symbols: REPA3
• NCBI Protein Information: replication protein A 14 kDa subunit; RP-A p14; RF-A protein 3; replication factor A protein 3
• UniProt Protein Name: Replication protein A 14 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA3
• UniProt Synonym Gene Names: REPA3; RPA14; RP-A p14; RF-A protein 3
• UniProt Entry Name: RFA3_HUMAN

Uniprot Description

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: nucleoplasm; DNA replication factor A complex

Molecular Function: protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle