Mouse anti-Human ROBO3 Monoclonal Antibody | anti-ROBO3 antibody

ROBO3 (Roundabout Homolog 3, Roundabout-like Protein 3, HGPS, HGPPS, RBIG1, RIG1) (PE)

Cat. Num. AAA6160005

• Gene Names: ROBO3; HGPS; RIG1; HGPPS; RBIG1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ROBO3; Monoclonal Antibody; ROBO3 (Roundabout Homolog 3; Roundabout-like Protein 3; HGPS; HGPPS; RBIG1; RIG1) (PE); anti-ROBO3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2H1
• Specificity: Recognizes human ROBO3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ROBO3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 0.1ng/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-148 from human ROBO3 (AAH08623) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDEEAWLPYSRPSFLSRGQGTSTCSTAGSNSSRGSSSSRGSRGPGRSRSQSRSQSQRPGQKRREEPR
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (42.28kD).)

Testing Data

(Detection limit for recombinant GST tagged ROBO3 is 0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-ROBO3 antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 14250380
• NCBI GeneID: 64221
• Molecular Weight: 110,676 Da
• NCBI Official Full Name: Homo sapiens roundabout, axon guidance receptor, homolog 3 (Drosophila), mRNA
• NCBI Official Synonym Full Names: roundabout guidance receptor 3
• NCBI Official Symbol: ROBO3
• NCBI Official Synonym Symbols: HGPS; RIG1; HGPPS; RBIG1
• NCBI Protein Information: roundabout homolog 3
• Protein Family: Roundabout

NCBI Description

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]