Immunohistochemistry - Paraffin (IHC)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human RIP1 / RALBP1 Monoclonal Antibody | anti-RIP1 / RALBP1 antibody
Anti-RIP1 / RALBP1 Antibody (clone OTI1H9) IHC-plus
IHC-P (10 ug/ml)
WB (1:2000)
Immunohistochemistry - Paraffin (IHC)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either pCMV6-ENTRY RALBP1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-RALBP1 mouse monoclonal, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either pCMV6-ENTRY RALBP1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-RALBP1 mouse monoclonal, and then analyzed by flow cytometry.)
NCBI and Uniprot Product Information
NCBI Description
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]
Uniprot Description
RALBP1: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis. Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
Protein type: GAPs; GAPs, Rac/Rho
Chromosomal Location of Human Ortholog: 18p11.3
Cellular Component: cytosol; membrane
Molecular Function: ATPase activity; ATPase activity, coupled to movement of substances; GTPase activator activity; protein binding; Rac GTPase binding; Ral GTPase binding; transmembrane transporter activity
Biological Process: chemotaxis; endocytosis; positive regulation of GTPase activity; regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction; small GTPase mediated signal transduction; transmembrane transport; transport
Research Articles on RIP1 / RALBP1
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Product Notes
The RIP1 / RALBP1 ralbp1 (Catalog #AAA249412) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-RIP1 / RALBP1 Antibody (clone OTI1H9) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RIP1 / RALBP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:100) IHC-P (10 ug/ml) WB (1:2000). Researchers should empirically determine the suitability of the RIP1 / RALBP1 ralbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIP1 / RALBP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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