Mouse anti-Human, rat Retinoic Acid Receptor, beta-Isotype Monoclonal Antibody | anti-RARB antibody

Anti-Retinoic Acid Receptor, beta-Isotype

Cat. Num. AAA500014

• Gene Names: RARB; HAP; RRB2; NR1B2; MCOPS12
• Reactivity: Human, rat
• Applications: Western Blot
• Purity: Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)
• Synonyms: Retinoic Acid Receptor; beta-Isotype; Monoclonal Antibody; Anti-Retinoic Acid Receptor; anti-RARB antibody

• Host: Mouse
• Reactivity: Human, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 336
• Specificity: Specific for the ~48k RAR-beta isotype.
• Purity/Purification: Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
• Sequence Length: 448

• Applicable Applications for anti-RARB antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Peptide corresponding to amino acids from N-terminal region of human retinoic acid receptor, beta-isotype. The antigen is identical in both beta1 and beta2 isoforms.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Canine, guinea pig, mouse and non-human primate
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in human and rat tissues. It is anticipated that the antibody will also work with canine, guinea pig, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The Retinoid X receptors (RXRs) family (RXRalpha, beta and gamma) preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors (RARs). RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005).
• Preparation and Storage: Store at -20 degree C; stable for at least 1 year.

Testing Data

Related Product Information for anti-RARB antibody

Affinity purified mouse monoclonal antibody

References

• Abu J, Batuwangala M, Herbert K, Symonds P (2005) Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. Lancet Oncol 6:712-720.
• Joshi S, Guleria R, Pan J, Dipette D, Singh US (2005) Retinoic acid receptors and tissue-trans-glutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene advance online publication 12 September 2005; doi: 10.1038/sj.onc.1209027.
• Lane MA, Bailey SJ (2005) Role of retinoid signalling in the adult brain. Prog Neurobiol 75:275-293.

NCBI and Uniprot Product Information

• NCBI GI #: 14916494
• NCBI GeneID: 5915
• NCBI Accession #: NP_000956.2
• NCBI GenBank Nucleotide #: NM_000965.3
• UniProt Accession #: P10826; P12891; Q00989; Q15298; Q9UN48
• Molecular Weight: 48
• NCBI Official Full Name: retinoic acid receptor beta isoform 1
• NCBI Official Synonym Full Names: retinoic acid receptor, beta
• NCBI Official Symbol: RARB
• NCBI Official Synonym Symbols: HAP; RRB2; NR1B2; MCOPS12
• NCBI Protein Information: retinoic acid receptor beta; RAR-beta; RAR-epsilon; HBV-activated protein; retinoic acid receptor beta 2; retinoic acid receptor beta 4; retinoic acid receptor beta 5; hepatitis B virus activated protein; retinoic acid receptor beta variant 1; retinoic acid receptor beta variant 2; retinoic acid receptor, beta polypeptide; nuclear receptor subfamily 1 group B member 2
• UniProt Protein Name: Retinoic acid receptor beta
• Protein Family: Retinoic acid receptor
• UniProt Gene Name: RARB
• UniProt Synonym Gene Names: HAP; NR1B2; RAR-beta
• UniProt Entry Name: RARB_HUMAN

NCBI Description

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Ref.10

Subunit structure: Homodimer

By similarity. Heterodimer; with a RXR molecule. Binds DNA preferentially as a RAR/RXR heterodimer

By similarity. Interacts weakly with NCOR2. Ref.10

Subcellular location: Isoform Beta-1: Nucleus. Isoform Beta-2: Nucleus. Isoform Beta-4: Cytoplasm.

Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Sequence similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain.

Sequence caution: The sequence CAA27637.1 differs from that shown. Reason: Erroneous gene model prediction.