Mouse anti-Human Renal Cell Carcinoma Marker, Aminopeptidase A Monoclonal Antibody
Renal Cell Carcinoma Marker, Aminopeptidase A
Reactivity
Human
Applications
Immunohistochemistry
Purity
PurifiedPurified
Synonyms
Renal Cell Carcinoma Marker; Aminopeptidase A; Monoclonal Antibody; Anti -Renal Cell Carcinoma Marker; anti-Renal Cell Carcinoma Marker; Aminopeptidase A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RC38
Specificity
Recognizes human Renal Cell Carcinoma. Stains 97% of primary renal cell carcinomas, 70% of metastatic renal cell cancers and proximal tubules in normal kidney. Does not stain epithelial cells of non-renal cell malignancies. Crossreactivity: endothelial cells in various tumors.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 10mM sodium azide, 1% BSA.
Applicable Applications for anti-Renal Cell Carcinoma Marker, Aminopeptidase A antibody
Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry (Frozen): 1:10 using the Indirect Immunoperoxidase method. Dilute in PBS.
Dilution: Immunohistochemistry (Frozen): 1:10 using the Indirect Immunoperoxidase method. Dilute in PBS.
Immunogen
Renal Cell Carcinoma
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Renal Cell Carcinoma Marker, Aminopeptidase A antibody
"Monoclonal antibody (MAb) RC38 recognizes a human renal antigen of 160 kD recently identified as human aminopeptidase A (APA; EC 3.4.11.7). This ectoenzyme is able to hydrolyse selectively N-terminal glutamyl and aspartyl residues from oligopeptides. By enzyme histochemistry, APA activity has also been localized in the microvessels of all organs in animals and man. A study was done to investigate the distribution of human APA as recognized by MAb RC38 in the microvasculature of normal human tissues and pathological conditions associated with neovascularization. Unexpectedly, in normal tissues vascular staining with MAb RC38 was generally weak and often absent, while in tumors, granulation tissue, and chronic synovitis, marked microvascular staining was demonstrated. By immuno-electron microscopy, the antigen was found on the cell membrane of activated pericytes and their processes in the tumor vasculature. RC38 expression could not be detected on cultured human endothelial cells or pericytes. These observations suggest that pericyte expression of a subtype of APA (as recognized by MAb RC38) is markedly enhanced in the vasculature of tumors and wound healing tissue as compared with normal resting tissues. This provides further evidence of the altered state of pericytes in these conditions. Pericyte APA may be involved in the metabolism of biologically active oligopeptides during neovascularization, supporting a regulatory role of pericytes in this process. In addition, MAb RC38 may be useful as a marker of pericyte activation in tissue sections.
Product Categories/Family for anti-Renal Cell Carcinoma Marker, Aminopeptidase A antibody
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Product Notes
The Renal Cell Carcinoma Marker, Aminopeptidase A (Catalog #AAA6004495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Renal Cell Carcinoma Marker, Aminopeptidase A reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Renal Cell Carcinoma Marker, Aminopeptidase A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry (Frozen): 1:10 using the Indirect Immunoperoxidase method. Dilute in PBS. Researchers should empirically determine the suitability of the Renal Cell Carcinoma Marker, Aminopeptidase A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Renal Cell Carcinoma Marker, Aminopeptidase A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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