Mouse anti-Human REN/Renin 1 Monoclonal Antibody | anti-REN antibody

Anti-REN/Renin 1 Antibody (aa131-406, clone 1D3)

Cat. Num. AAA2401383

• Gene Names: REN; HNFJ2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: REN/Renin 1; Monoclonal Antibody; Anti-REN/Renin 1 Antibody (aa131-406; clone 1D3); Sheep Polyclonal to Human REN/Renin 1; REN; Angiotensin-forming enzyme; Angiotensinogenase; HNFJ2; RENAA; Renin precursor; renal; Renin; anti-REN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1D3
• Specificity: Human REN/Renin 1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Sequence Length: 406

• Applicable Applications for anti-REN antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 10 ug/ml; WB: 1:500
• Immunogen: Human recombinant protein fragment corresponding to amino acids 131-406 of human REN(NP_000528) produced in E. coli.
• Conjugation: Unconjugated
• Epitope: aa131-406
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY REN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-REN (1:500).)

Related Product Information for anti-REN antibody

Renin catalyzes the first step in the activation pathway ofangiotensinogen--a cascade that can result in aldosteronerelease, vasoconstriction, and increase in blood pressure. Renin, anaspartyl protease, cleaves angiotensinogen to form angiotensin I, whichis converted to angiotensin II by angiotensin I converting enzyme, animportant regulator of blood pressure and electrolyte balance.

Product Categories/Family for anti-REN antibody

Protease; Aspartic A1

NCBI and Uniprot Product Information

• NCBI GI #: 4506475
• NCBI GeneID: 5972
• NCBI Accession #: NP_000528.1
• NCBI GenBank Nucleotide #: NM_000537.3
• UniProt Accession #: P00797; Q6FI38; Q6T5C2
• Molecular Weight: 44,726 Da
• NCBI Official Full Name: renin preproprotein
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin
• UniProt Protein Name: Renin
• Protein Family: Renin
• UniProt Gene Name: REN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.