Mouse anti-Human RBP1 / CRBP Monoclonal Antibody | anti-RBP1 antibody

Mouse Monoclonal [clone 2E1] (IgG2a) to Human RBP1 / CRBP

Cat. Num. AAA249005

• Gene Names: RBP1; CRBP; RBPC; CRBP1; CRBPI; CRABP-I
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G Purified
• Synonyms: RBP1 / CRBP; Monoclonal Antibody; Mouse Monoclonal [clone 2E1] (IgG2a) to Human RBP1 / CRBP; Anti-RBP1 / CRBP Antibody (clone 2E1) IHC-plus; RBP1; CRABP-I; CRBP; CRBP-I; RBPC; Retinol-binding protein 1; CRBP1; CRBPI; Human RBP1; anti-RBP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2E1
• Specificity: Human CRBP1 / CRBP
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.94 mg/ml (varies by lot)
• Sequence Length: 197

• Applicable Applications for anti-RBP1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:100), IF (1:100), IHC-P (10 ug/ml), WB (1:2000)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human RBP1(NP_002890) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: RBP1 / CRBP antibody was raised against full length human recombinant protein of human RBP1(NP_002890) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RBP1 / CRBP antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Anti-RBP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RBP1.)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RBP1 antibody, and then analyzed by flow cytometry.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBP1.)

NCBI and Uniprot Product Information

• NCBI GI #: 195976807
• NCBI GeneID: 5947
• NCBI Accession #: NP_002890.2
• NCBI GenBank Nucleotide #: NM_002899.3
• UniProt Accession #: P09455; Q6FGX8; A8K2Q0; B7Z7A0; E7EWV0; F2Z2F2
• Molecular Weight: 17,523 Da
• NCBI Official Full Name: retinol-binding protein 1 isoform a
• NCBI Official Synonym Full Names: retinol binding protein 1, cellular
• NCBI Official Symbol: RBP1
• NCBI Official Synonym Symbols: CRBP; RBPC; CRBP1; CRBPI; CRABP-I
• NCBI Protein Information: retinol-binding protein 1; CRBP-I; cellular retinol-binding protein I; retinol-binding protein 1, cellular
• UniProt Protein Name: Retinol-binding protein 1
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBP1
• UniProt Synonym Gene Names: CRBP1; CRBP; CRBP-I
• UniProt Entry Name: RET1_HUMAN

NCBI Description

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

RBP1: Intracellular transport of retinol. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: cytosol

Molecular Function: retinoid binding; transporter activity; retinol binding; retinal binding

Biological Process: phototransduction, visible light; transport; retinoid metabolic process; vitamin A metabolic process