Mouse RBFOX1 Monoclonal Antibody | anti-RBFOX1 antibody

RBFOX1 Antibody

Cat. Num. AAA415053

• Gene Names: RBFOX1; 2BP1; FOX1; A2BP1; FOX-1; HRNBP1
• Reactivity: Human, cow, mouse and rat
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: RBFOX1; Monoclonal Antibody; RBFOX1 Antibody; anti-RBFOX1 antibody

• Host: Mouse
• Reactivity: Human, cow, mouse and rat
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 397

• Applicable Applications for anti-RBFOX1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: The antibody solution can be used at dilutions of 1:500-1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000-2,000.
• Immunogen: Fox1 is one of a family of 3 mammalian Fox1 homologues. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X" (1). The C. elegans Fox1 protein and its 3 mammalian homologues are all about 46kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2). As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3). Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5). Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6). Our antibody was raised against the N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli. We did not use full length Fox1 as immunogen since the three mammalian Fox1 homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated. The HGNC name for this protein is RBFOX1.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

References

1. Hodgkin J, Zellan JD, Albertson DG. Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans. Development 120:3681-3689 (1994).2. Shibata H, Huynh DP, Pulst SM. A novel protein with RNA-binding motifs interacts with ataxin-2. Hum Mol Genet. 39:1303-13 (2000).3. Underwood JG, Boutz PL, Dougherty JD, Stoilov P and Black DL. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol. Cell. Biol. 25:10005-10016 (2005).4. Mullen RJ, Buck CR, Smith AM. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116:201-211 (1994).5. Kim KK, Adelstein RS, Kawamoto S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284:31052-31061 (2009).6. Kim KK, Kim YC, Adelstein RS, Kawamoto S. Fox-3 and PSF interact to activate neural cell-specific alternative splicing. Nucleic Acids Res. 39:3064-78 (2011).

NCBI and Uniprot Product Information

• NCBI GI #: 215272412
• NCBI GeneID: 54715
• NCBI Accession #: NP_001135806.1
• NCBI GenBank Nucleotide #: NM_001142334.1
• UniProt Accession #: Q9NWB1; Q7Z7I7; Q8TAE3; Q8TAF2; Q8WYB2; Q9NS20
• Molecular Weight: 42,784 Da
• NCBI Official Full Name: RNA binding protein fox-1 homolog 1 isoform 4
• NCBI Official Synonym Full Names: RNA binding protein, fox-1 homolog (C. elegans) 1
• NCBI Official Symbol: RBFOX1
• NCBI Official Synonym Symbols: 2BP1; FOX1; A2BP1; FOX-1; HRNBP1
• NCBI Protein Information: RNA binding protein fox-1 homolog 1; fox-1 homolog A; ataxin 2-binding protein 1; hexaribonucleotide-binding protein 1
• UniProt Protein Name: RNA binding protein fox-1 homolog 1
• Protein Family: RNA binding protein
• UniProt Gene Name: RBFOX1
• UniProt Synonym Gene Names: A2BP; A2BP1; FOX1; HRNBP1
• UniProt Entry Name: RFOX1_HUMAN

NCBI Description

The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

Function: RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. Ref.7

Subunit structure: Binds to the C-terminus of ATXN2. Ref.1

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity.

Tissue specificity: Predominantly expressed in muscle and brain.

Sequence similarities: Contains 1 RRM (RNA recognition motif) domain.

Sequence caution: The sequence AAF78291.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.