Mouse RBC Monoclonal Antibody | anti-RBC antibody
RBC, Mouse, mAb 34-3C
Reactivity
Rat: No; Sheep: No; Rabbit: No; Chicken: No; Human: No
Applications
Flow Cytometry
Purity
Protein G
Synonyms
RBC; Monoclonal Antibody; Mouse; mAb 34-3C; anti-RBC antibody
Host
Mouse
Reactivity
Rat: No; Sheep: No; Rabbit: No; Chicken: No; Human: No
Clonality
Monoclonal
Isotype
Mouse IgG2a
Purity/Purification
Protein G
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Concentration
100 ug/ml (varies by lot)
Applicable Applications for anti-RBC antibody
Flow Cytometry (FC), Functional Studies (FS)
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical working dilution is 1:50.
-Positive control: Mouse erythrocytes.
-Positive control: Mouse erythrocytes.
Immunogen
RBC
Endotoxin
<0.4 EU/mg
Positive Control
Mouse erythrocytes
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Related Product Information for anti-RBC antibody
Product Description: The monoclonal antibody 34-3C recognizes an exposed surface determinant of intact red blood cells (RBC). The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity. The monoclonal antibody only recognises antigenic determinants expressed on Mouse RBC and not on other species of RBC.
References
1. Shibata, T et al; Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune haemolytic anemia by two distinct pathogenic mechanisms. Int. Immun. 1990, 2:1133 2. Fossati-Jimack, L et al; High pathogenic potential of low-affinity autoantibodies in experimental autoimmune haemolytic anemia. J. Exp. Med. 1990, 190:1689
3. Fossati-Jimack, L et al; Selective increase of autoimmune epitope expression on aged erythrocytes in mice: Implications in anti-erythrocyte autoimmune responses. J. Autoimmun. 2002, 18:17
4. Azeredo da Silveira, S et al; Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high-affinity anti-erythrocyte autoantibody. J. Exp. Med. 2002, 195:665
5. Baudino, L et al; Differential contribution of three different activating IgG Fc receptors (FcyRI, FcyRIII and FcyRIV) to IgG2a- and IgG2b-induced autoimmune haemolytic anemia in mice. J. Immunol. 2008, 180:1948
3. Fossati-Jimack, L et al; Selective increase of autoimmune epitope expression on aged erythrocytes in mice: Implications in anti-erythrocyte autoimmune responses. J. Autoimmun. 2002, 18:17
4. Azeredo da Silveira, S et al; Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high-affinity anti-erythrocyte autoantibody. J. Exp. Med. 2002, 195:665
5. Baudino, L et al; Differential contribution of three different activating IgG Fc receptors (FcyRI, FcyRIII and FcyRIV) to IgG2a- and IgG2b-induced autoimmune haemolytic anemia in mice. J. Immunol. 2008, 180:1948
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Product Notes
The RBC (Catalog #AAA584284) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RBC, Mouse, mAb 34-3C reacts with Rat: No; Sheep: No; Rabbit: No; Chicken: No; Human: No and may cross-react with other species as described in the data sheet. AAA Biotech's RBC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Functional Studies (FS). Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical working dilution is 1:50. -Positive control: Mouse erythrocytes. Researchers should empirically determine the suitability of the RBC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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