Testing Data
Rat Anti-Mouse Kappa (kappa chain specific) Secondary Antibody
Rat Anti-Mouse Kappa-PE
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence
Synonyms
Rat Anti-Mouse Kappa (kappa chain specific); Monoclonal Secondary Antibody; Rat Anti-Mouse Kappa-PE; Rat Anti-Mouse Kappa - Phycoerythrin-Labeled; Rat Anti-Mouse Kappa (kappa chain specific) secondary antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
H139-52.1
Specificity
Reacts with mouse kappa light chains
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for Rat Anti-Mouse Kappa (kappa chain specific) secondary antibody
ELISA; Flow Cytometry; Immunofluorescence
Application Notes
Research Application(s): Identification and enumeration of kappa ^+ cells by flow cytometry
Identification and enumeration of kappa ^+ cells by immunofluorescence microscopy
Flow Cytometry Suggested Dilution: <=0.2 ug/10^6 cells
Identification and enumeration of kappa ^+ cells by immunofluorescence microscopy
Flow Cytometry Suggested Dilution: <=0.2 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by ELISA and/or flow cytometry to conform to characteristics of a standard reference. Representative data are included in this data sheet.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Testing Data
Testing Data
Product Categories/Family for Rat Anti-Mouse Kappa (kappa chain specific) secondary antibody
Similar Products
Product Notes
The Rat Anti-Mouse Kappa (kappa chain specific) (Catalog #AAA673062) is a Secondary Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse Kappa-PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rat Anti-Mouse Kappa (kappa chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunofluorescence. Research Application(s): Identification and enumeration of kappa ^+ cells by flow cytometry Identification and enumeration of kappa ^+ cells by immunofluorescence microscopy Flow Cytometry Suggested Dilution: <=0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the Rat Anti-Mouse Kappa (kappa chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rat Anti-Mouse Kappa (kappa chain specific), Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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