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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RASD2 protein (red) or empty vector (blue) stained using RASD2 antibody)

Mouse RASD2 Monoclonal Antibody | anti-RASD2 antibody

RASD2 antibody

Gene Names
RASD2; Rhes; TEM2; MGC:4834
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
RASD2 antibody was purified by affinity chromatography.
Synonyms
RASD2; Monoclonal Antibody; RASD2 antibody; Monoclonal RASD2; Anti-RASD2; RASD family member 2; TEM2; Rhes; Tumor endothelial marker 2; Ras homolog enriched in striatum; GTP-binding protein Rhes; RASD 2; RASD-2; anti-RASD2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1H10
Purity/Purification
RASD2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
266
Applicable Applications for anti-RASD2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
Biological Significance
RASD2 is a Ras-related protein that is enriched in striatum. The product of this gene binds to GTP and possessesintrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades.
Cross-Reactivity
Human
Immunogen
RASD2 antibody was raised in mouse using a full length recombinant protein of human RASD2 (NP_055125) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RASD2 protein (red) or empty vector (blue) stained using RASD2 antibody)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RASD2 protein (red) or empty vector (blue) stained using RASD2 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RASD2 protein (Right) or empty vector (Left) detected with RASD2 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RASD2 protein (Right) or empty vector (Left) detected with RASD2 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RASD2 protein using RASD2 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RASD2 protein using RASD2 antibody)
Related Product Information for anti-RASD2 antibody
Mouse monoclonal RASD2 antibody
Product Categories/Family for anti-RASD2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,366 Da
NCBI Official Full Name
GTP-binding protein Rhes
NCBI Official Synonym Full Names
RASD family, member 2
NCBI Official Symbol
RASD2
NCBI Official Synonym Symbols
Rhes; TEM2; MGC:4834
NCBI Protein Information
GTP-binding protein Rhes
UniProt Protein Name
GTP-binding protein Rhes
Protein Family
UniProt Gene Name
RASD2
UniProt Synonym Gene Names
TEM2
UniProt Entry Name
RHES_HUMAN

NCBI Description

This gene encodes a Ras-related protein that enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades. [provided by RefSeq, Jul 2008]

Uniprot Description

RASD2: GTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination. Belongs to the small GTPase superfamily. RasD family.

Protein type: G protein, monomeric; G protein, monomeric, RasD; G protein

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: plasma membrane

Molecular Function: GTPase activity; GTP binding; ubiquitin conjugating enzyme binding; G-protein beta-subunit binding

Biological Process: positive regulation of protein sumoylation; positive regulation of protein kinase B signaling cascade; synaptic transmission, dopaminergic; metabolic process; small GTPase mediated signal transduction; negative regulation of protein ubiquitination; locomotory behavior

Research Articles on RASD2

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Product Notes

The RASD2 rasd2 (Catalog #AAA834766) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RASD2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the RASD2 rasd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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