Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate The Recombinant protein (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : KRAS recombinant protein (ATGP2062) Lane 2. : HRAS recombinant protein (ATGP0516) Lane 3. : NRAS recombinant protein (ATGP0492))
Mouse anti-Human RAS Monoclonal Antibody | anti-RAS antibody
RAS antibody
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate The Recombinant protein (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : KRAS recombinant protein (ATGP2062) Lane 2. : HRAS recombinant protein (ATGP0516) Lane 3. : NRAS recombinant protein (ATGP0492))
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate The Recombinant protein (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : KRAS recombinant protein (ATGP2062) Lane 2. : HRAS recombinant protein (ATGP0516) Lane 3. : NRAS recombinant protein (ATGP0492))
Immunofluorescence (IF)
(ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Uniprot Description
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
Research Articles on RAS
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Product Notes
The RAS kras (Catalog #AAA200363) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAS antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the RAS kras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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