Mouse anti-Human, Mouse Raptor Monoclonal Antibody | anti-RPTOR antibody

Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats, KIAA1303) (MaxLight 405)

Cat. Num. AAA6245147

• Gene Names: RPTOR; KOG1; Mip1; KIAA1303
• Reactivity: Human, Mouse
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Raptor; Monoclonal Antibody; Raptor (Regulatory Associated Protein of mTOR; p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats; KIAA1303) (MaxLight 405); anti-RPTOR antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 10F657
• Specificity: Recognizes human Raptor at ~150kD. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-RPTOR antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Recombinant corresponding to human Raptor
• Conjugate: MaxLight405
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Confocal fluorescent analysis of NIH/3T3 cells using MBS605927 (Red) (1:500). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)

Testing Data

(HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS605927 (1ug/ml). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates Raptor (~150kD).)

Product Categories/Family for anti-RPTOR antibody

Antibodies; Protein Kinase Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 22094987
• NCBI GeneID: 57521
• NCBI Accession #: NP_065812.1
• NCBI GenBank Nucleotide #: NM_020761.2
• UniProt Accession #: Q8N122; Q8N4V9; Q8TB32; Q9P2P3; B2RN36; Q6DKI0
• Molecular Weight: 149,038 Da
• NCBI Official Full Name: regulatory-associated protein of mTOR isoform 1
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: RPTOR
• NCBI Official Synonym Symbols: KOG1; Mip1; KIAA1303
• NCBI Protein Information: regulatory-associated protein of mTOR; raptor; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
• UniProt Protein Name: Regulatory-associated protein of mTOR
• Protein Family: Regulatory-associated protein
• UniProt Gene Name: RPTOR
• UniProt Synonym Gene Names: KIAA1303; RAPTOR; Raptor

Uniprot Description

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.