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Testing Data (Detection limit for recombinant GST tagged RAP1A is ~1ng/ml as a capture antibody.)

Mouse anti-Human RAP1A Monoclonal Antibody | anti-RAP1A antibody

RAP1A (Ras-related Protein Rap-1A, C21KG, G-22K, GTP-binding Protein smg p21A, Ras-related Protein Krev-1, KREV1) (PE)

Gene Names
RAP1A; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RAP1A; Monoclonal Antibody; RAP1A (Ras-related Protein Rap-1A; C21KG; G-22K; GTP-binding Protein smg p21A; Ras-related Protein Krev-1; KREV1) (PE); anti-RAP1A antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E1
Specificity
Recognizes human RAP1A.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-RAP1A antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to human RAP1A, aa1-185 (AAH14086) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged RAP1A is ~1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged RAP1A is ~1ng/ml as a capture antibody.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between RASGRP4 and RAP1A HeLa cells were stained with RASGRP4 rabbit purified polyclonal 1:1200 and RAP1A mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Testing Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between RASGRP4 and RAP1A HeLa cells were stained with RASGRP4 rabbit purified polyclonal 1:1200 and RAP1A mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Product Categories/Family for anti-RAP1A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
20,987 Da
NCBI Official Full Name
Homo sapiens RAP1A, member of RAS oncogene family, mRNA
NCBI Official Synonym Full Names
RAP1A, member of RAS oncogene family
NCBI Official Symbol
RAP1A
NCBI Official Synonym Symbols
RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
NCBI Protein Information
ras-related protein Rap-1A
Protein Family

NCBI Description

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Research Articles on RAP1A

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Product Notes

The RAP1A (Catalog #AAA6159825) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAP1A (Ras-related Protein Rap-1A, C21KG, G-22K, GTP-binding Protein smg p21A, Ras-related Protein Krev-1, KREV1) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RAP1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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