Mouse anti-Human RALBP1 Monoclonal Antibody | anti-RALBP1 antibody

RALBP1 (RLIP1, RLIP76, RalA-binding Protein 1, 76kD Ral-interacting Protein, Dinitrophenyl S-glutathione ATPase, DNP-SG ATPase, Ral-interacting Protein 1) APC

Cat. Num. AAA6138606

• Gene Names: RALBP1; RIP1; RLIP1; RLIP76
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RALBP1; Monoclonal Antibody; RALBP1 (RLIP1; RLIP76; RalA-binding Protein 1; 76kD Ral-interacting Protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting Protein 1) APC; anti-RALBP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2A1
• Specificity: Recognizes human RALBP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RALBP1 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-656 from human RALBP1 (AAH13126) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTECFLPPTSSPSEHRRVEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDILHEPPDVVSDDEKDHGKKKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKMKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEKHKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQIDVPNLKPIFGIPLADAVERTMMYDGIRLPAVFRECIDYVEKYGMKCEGIYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRDLPENLLTKELMPRFEEACGRTTETEKVQEFQRLLKELPECNYLLISWLIVHMDHVIAKELETKMNIQNISIVLSPTVQISNRVLYVFFTHVQELFGNVVLKQVMKPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAREQPKAGKEPAKPSPSRDRKETSI
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (98.16kD).)

Western Blot (WB)

(RALBP1 monoclonal antibody, Western Blot analysis of RALBP1 expression in HeLa.)

Western Blot (WB)

(RALBP1 monoclonal antibody Western Blot analysis of RALBP1 expression in C32.)

Immunoprecipitation (IP)

(Immunoprecipitation of RALBP1 transfected lysate using RALBP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with RALBP1 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged RALBP1 is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-RALBP1 antibody

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).

Product Categories/Family for anti-RALBP1 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15341886
• NCBI GeneID: 10928
• Molecular Weight: 76,063 Da
• NCBI Official Full Name: Homo sapiens ralA binding protein 1, mRNA
• NCBI Official Synonym Full Names: ralA binding protein 1
• NCBI Official Symbol: RALBP1
• NCBI Official Synonym Symbols: RIP1; RLIP1; RLIP76
• NCBI Protein Information: ralA-binding protein 1
• Protein Family: RalA-binding protein

NCBI Description

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]