Rat RalB Monoclonal Antibody | anti-RALB antibody

RalB (Ras-related Protein)

Cat. Num. AAA601799

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: RalB; Monoclonal Antibody; RalB (Ras-related Protein); Anti -RalB (Ras-related Protein); anti-RALB antibody

• Host: Rat
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7H220
• Specificity: Recognizes endogenous human RalB at ~25kD. Species Crossreactivity: mouse and rat. Does not crossreact with recombinant human RalA.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-RALB antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Full-length recombinant protein corresponding to aa1-206 from human RalB expressed in E. coli. (P11234).
• Hybridoma: Myeloma cells with rat B cells.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RALB antibody

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Product Categories/Family for anti-RALB antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4506405
• NCBI GeneID: 5899
• NCBI Accession #: NP_002872.1
• NCBI GenBank Nucleotide #: NM_002881.2
• UniProt Accession #: P11234; Q53T32; Q6ZS74
• Molecular Weight: 23,409 Da
• NCBI Official Full Name: ras-related protein Ral-B
• NCBI Official Synonym Full Names: v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
• NCBI Official Symbol: RALB
• NCBI Protein Information: ras-related protein Ral-B; OTTHUMP00000162132; OTTHUMP00000204385; OTTHUMP00000207262; RAS-like protein B; GTP binding protein
• UniProt Protein Name: Ras-related protein Ral-B
• Protein Family: Ras-related protein
• UniProt Gene Name: RALB
• UniProt Entry Name: RALB_HUMAN

NCBI Description

This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. [provided by RefSeq]

Uniprot Description

RALB: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis. Interacts with EXOC2 and EXOC8. Interacts with RALBP1 via its effector domain. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric; G protein, monomeric, Ras; G protein

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: exocyst; plasma membrane; autophagic vacuole; midbody

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; ubiquitin protein ligase binding; ATPase binding

Biological Process: regulation of exocyst localization; nerve growth factor receptor signaling pathway; positive regulation of protein binding; apoptosis; metabolic process; regulation of exocyst assembly; Ras protein signal transduction; cytokinesis; positive regulation of protein amino acid phosphorylation; signal transduction; negative regulation of protein binding; cellular response to starvation