Mouse anti-Human Raf Monoclonal Antibody
Raf, phosphorylated (Ser621) (Raf-1, c-Raf)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified
Synonyms
Raf; Monoclonal Antibody; phosphorylated (Ser621) (Raf-1; c-Raf); Anti -Raf; anti-Raf antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6D720
Specificity
Recognizes human c-Raf phosphorylated at Ser621.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Applicable Applications for anti-Raf antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA, Western Blotting.
Dilution: Western Blotting: 1:100-1:1000
Dilution: Western Blotting: 1:100-1:1000
Immunogen
Synthetic peptide corresponding to a portion of human c-Raf phosphorylated at Ser621.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50?. Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Raf antibody
The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf and c-Raf) that play a critical role in regulating cell growth and differentiation and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74kD signal transducer of multiple extracellular stimuli that is regulated by several pathways and that once activated phosphorylates MEK which in turn phosphorylates ERK. Together with serine 43 and serine 259, serine 621 is one of the three constitutive phosphorylation sites of c-Raf in resting cells. Phosphorylation of serine 621 has been shown to positively or negatively regulate c-Raf kinase activity, depending on the system.
Product Categories/Family for anti-Raf antibody
NCBI and Uniprot Product Information
Uniprot Description
Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.
Similar Products
Product Notes
The Raf (Catalog #AAA609994) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Raf, phosphorylated (Ser621) (Raf-1, c-Raf) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Raf can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA, Western Blotting. Dilution: Western Blotting: 1:100-1:1000. Researchers should empirically determine the suitability of the Raf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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