Western Blot (WB)
(All lanes : Anti-RAB11A Antibody at1:4000 dilutionLane 1: 293T/17 whole cell lysateLane 2: C2C12 whole cell lysateLane 3: Hela whole cell lysateLane 4: Jurkat whole cell lysateLane 5: PC-12 whole cell lysateLane 6: rat brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse RAB11A Monoclonal Antibody | anti-RAB11A antibody
RAB11A Antibody
Gene Names
RAB11A; YL8
Reactivity
Human, Mouse, RatPredicted: Pig, Rabbit
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
RAB11A; Monoclonal Antibody; RAB11A Antibody; Ras-related protein Rab-11A; Rab-11; YL8; RAB11; anti-RAB11A antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Predicted: Pig, Rabbit
Predicted: Pig, Rabbit
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This RAB11A antibody is generated from a mouse immunized with a purified recombinant protein of human RAB11A.
Sequence Length
155
Applicable Applications for anti-RAB11A antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:4000
IHC-P: 1:25
IHC-P: 1:25
Antigen Source
Human
Antigen Region
1-216
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-RAB11A Antibody at1:4000 dilutionLane 1: 293T/17 whole cell lysateLane 2: C2C12 whole cell lysateLane 3: Hela whole cell lysateLane 4: Jurkat whole cell lysateLane 5: PC-12 whole cell lysateLane 6: rat brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-RAB11A Antibody at1:4000 dilutionLane 1: 293T/17 whole cell lysateLane 2: C2C12 whole cell lysateLane 3: Hela whole cell lysateLane 4: Jurkat whole cell lysateLane 5: PC-12 whole cell lysateLane 6: rat brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(Staining RAB11A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(Staining RAB11A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Related Product Information for anti-RAB11A antibody
Description: Purified Mouse Monoclonal Antibody (Mab)
Background: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
Background: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
Product Categories/Family for anti-RAB11A antibody
References
Drivas G.T.,et al.Oncogene 6:3-9(1991). Zahraoui A.,et al.Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases. Gromov P.S.,et al.FEBS Lett. 429:359-364(1998). Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases. Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24394
NCBI Official Full Name
ras-related protein Rab-11A isoform 2
NCBI Official Synonym Full Names
RAB11A, member RAS oncogene family
NCBI Official Symbol
RAB11A
NCBI Official Synonym Symbols
YL8
NCBI Protein Information
ras-related protein Rab-11A
UniProt Protein Name
Ras-related protein Rab-11A
Protein Family
UniProt Gene Name
RAB11A
UniProt Synonym Gene Names
RAB11; Rab-11
UniProt Entry Name
RB11A_HUMAN
NCBI Description
The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Research Articles on RAB11A
Similar Products
Product Notes
The RAB11A rab11a (Catalog #AAA9232199) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB11A Antibody reacts with Human, Mouse, Rat Predicted: Pig, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's RAB11A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:4000 IHC-P: 1:25. Researchers should empirically determine the suitability of the RAB11A rab11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB11A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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