Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human QPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : K562 cell lysate )
Mouse anti-Human QPRT Monoclonal Antibody | anti-QPRT antibody
QPRT antibody
Gene Names
QPRT; QPRTase; HEL-S-90n
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
QPRT; Monoclonal Antibody; QPRT antibody; Nicotinate nucleotide pyrophosphorylase (carboxylating); Nicotinate nucleotide pyrophosphorylase carboxylating; Quinolinate phosphoribosyltransferase; QPRTase; QAPRTase; anti-QPRT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT24C4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
297
Applicable Applications for anti-QPRT antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human QPRT (1-297aa) purified from E. coli
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week.
For long term storage, aliquot and store at -20C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20C to -80°C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human QPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : K562 cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human QPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : K562 cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of QPRT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human QPRT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of QPRT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human QPRT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-QPRT antibody
Quinolinate phosphoribosyltransferase, also known as QPRT, is a major enzyme in the catabolism of quinolinate. It is an intermediate in the tryptophannicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide and pyrophosphate. Elevation of QPRT levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.
Product Categories/Family for anti-QPRT antibody
References
Cao H., et al. (2002) Biochemistry. 41 (10): 3520-8.; Liu H., et al. (2007) J Mol Biol. 373 (3): 755-63.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nicotinate-nucleotide pyrophosphorylase
NCBI Official Synonym Full Names
quinolinate phosphoribosyltransferase
NCBI Official Symbol
QPRT
NCBI Official Synonym Symbols
QPRTase; HEL-S-90n
NCBI Protein Information
nicotinate-nucleotide pyrophosphorylase [carboxylating]
UniProt Protein Name
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Protein Family
UniProt Gene Name
QPRT
UniProt Synonym Gene Names
QAPRTase; QPRTase
NCBI Description
This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Uniprot Description
Involved in the catabolism of quinolinic acid (QA).
Research Articles on QPRT
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Product Notes
The QPRT qprt (Catalog #AAA200411) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The QPRT antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's QPRT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the QPRT qprt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "QPRT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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