Mouse PUF60 Monoclonal Antibody | anti-PUF60 antibody

PUF60 (poly-U Binding Splicing Factor 60kD, FIR, FLJ31379, RoBPI, SIAHBP1) (APC)

Cat. Num. AAA6169290

• Gene Names: PUF60; FIR; VRJS; RoBPI; SIAHBP1
• Applications: Immunofluorescence, Western Blot
• Purity: Purified
• Synonyms: PUF60; Monoclonal Antibody; PUF60 (poly-U Binding Splicing Factor 60kD; FIR; FLJ31379; RoBPI; SIAHBP1) (APC); poly-U Binding Splicing Factor 60kD; SIAHBP1; anti-PUF60 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C1
• Specificity: Recognizes PUF60.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 516

• Applicable Applications for anti-PUF60 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PUF60 (AAH11979, 1aa-516aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1.Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa).Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PUF60 on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PUF60 on HeLa cell. [antibody concentration 10 ug/ml])

Related Product Information for anti-PUF60 antibody

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Product Categories/Family for anti-PUF60 antibody

Antibodies; Splicing Factors

NCBI and Uniprot Product Information

• NCBI GI #: 15080471
• NCBI GeneID: 22827
• NCBI Official Full Name: PUF60 protein
• NCBI Official Synonym Full Names: poly(U) binding splicing factor 60
• NCBI Official Symbol: PUF60
• NCBI Official Synonym Symbols: FIR; VRJS; RoBPI; SIAHBP1
• NCBI Protein Information: poly(U)-binding-splicing factor PUF60
• Protein Family: Poly(U)-binding-splicing factor

NCBI Description

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]