Western Blot (WB)
(Western BlotPositive WB detected in?Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)All lanes?Phospho-PTPN11 antibody at 0.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 68 KDaObserved band size: 68 KDa)
PTPN11 recombinant antibody
Phospho-PTPN11 (Y542) Antibody
IP: 1:200-1:1000
Western Blot (WB)
(Western BlotPositive WB detected in?Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)All lanes?Phospho-PTPN11 antibody at 0.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 68 KDaObserved band size: 68 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in?Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)All lanes?Phospho-PTPN11 antibody at 0.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 68 KDaObserved band size: 68 KDa)
Immunoprecipitation (IP)
(Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with PervanadateLane 1: Rabbit control IgG(1ug)instead of CSB-RA019025A542phHU in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA019025A542phHU(3ug)+ Hela whole cell lysate treated with Pervanadate(1mg)Lane 3: Hela whole cell lysate treated with Pervanadate(20ug))
Immunoprecipitation (IP)
(Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with PervanadateLane 1: Rabbit control IgG(1ug)instead of CSB-RA019025A542phHU in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA019025A542phHU(3ug)+ Hela whole cell lysate treated with Pervanadate(1mg)Lane 3: Hela whole cell lysate treated with Pervanadate(20ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]
Uniprot Description
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).
Research Articles on PTPN11
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Product Notes
The PTPN11 ptpn11 (Catalog #AAA7114888) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PTPN11 (Y542) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:5000 IP: 1:200-1:1000. Researchers should empirically determine the suitability of the PTPN11 ptpn11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN11, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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