Mouse anti-Human PSME2 Monoclonal Antibody | anti-PSME2 antibody

Anti-PSME2 Antibody, Mouse Monoclonal

Cat. Num. AAA8107452

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: PSME2; Monoclonal Antibody; Anti-PSME2 Antibody; Mouse Monoclonal; PSME2/PA28b Antibody; Mouse MAb; proteasome (prosome; macropain) activator subunit 2 (PA28 beta); Anti-PA28B Antibody; Anti-PA28beta Antibody; Anti-REGbeta Antibody; anti-PSME2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 06
• Specificity: Human PSME2/PA28b.; No cross-reactivity in ELISA with E Coli cell lysate
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PSME2 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human PSME2/PA28b protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSME2/PA28b (rh PSME2/PA28b). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSME2 mouse monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:30 kDa)

Immunoprecipitation (IP)

(PSME2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-PSME2 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PSME2 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa)

Related Product Information for anti-PSME2 antibody

PSME2, also known as PA28b, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring.

References

Ahn K. et al., 1996, J Biol Chem. 271 (30): 18237-42. Rual Jean-Francois. et al., 2005, Nature. 437 (7062): 1173-8. Ahn JY. et al., 1995, FEBS Lett. 366 (1): 37-42.