Mouse PSMD10 Monoclonal Antibody | anti-PSMD10 antibody

PSMD10 (Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 10, dJ889N15.2, p28) (PE)

Cat. Num. AAA6186787

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Applications: ELISA, Western Blot
• Purity: Purified
• Synonyms: PSMD10; Monoclonal Antibody; PSMD10 (Proteasome (Prosome; Macropain) 26S Subunit; non-ATPase; 10; dJ889N15.2; p28) (PE); Proteasome (Prosome; p28; anti-PSMD10 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F10
• Specificity: Recognizes PSMD10.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 226

• Applicable Applications for anti-PSMD10 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PSMD10 (AAH11960, 1aa-226aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PSMD10 expression in transfected 293T cell line by PSMD10 monoclonal antibody (M19), clone 3F10.Lane 1: PSMD10 transfected lysate (24.4 KDa).Lane 2: Non-transfected lysate.)

Related Product Information for anti-PSMD10 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20. [provided by RefSeq]

Product Categories/Family for anti-PSMD10 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 15080417
• NCBI GeneID: 5716
• NCBI Official Full Name: Proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]