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Western Blot (WB) (PSMA1 monoclonal antibody Western Blot analysis of PSMA1 expression in HeLa.)

Mouse anti-Human PSMA1 Monoclonal Antibody | anti-PSMA1 antibody

PSMA1 (Proteasome Subunit alpha Type-1, 30kD Prosomal Protein, PROS-30, Macropain Subunit C2, Multicatalytic Endopeptidase Complex Subunit C2, Proteasome Component C2, Proteasome nu Chain, HC2, NU, PROS30, PSC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC2

Gene Names
PSMA1; NU; HC2; PROS30; HEL-S-275
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PSMA1; Monoclonal Antibody; PSMA1 (Proteasome Subunit alpha Type-1; 30kD Prosomal Protein; PROS-30; Macropain Subunit C2; Multicatalytic Endopeptidase Complex Subunit C2; Proteasome Component C2; Proteasome nu Chain; HC2; NU; PROS30; PSC2; MGC14542; MGC14575; MGC14751; MGC1667; MGC2; anti-PSMA1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1D9-1C7
Specificity
Recognizes human PSMA1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-PSMA1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 60ug/ml
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to human PSMA1, aa1-264 (AAH02577) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSMA1 monoclonal antibody Western Blot analysis of PSMA1 expression in HeLa.)

Western Blot (WB) (PSMA1 monoclonal antibody Western Blot analysis of PSMA1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody. Lane 1: PSMA1 transfected lysate (30.2kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody. Lane 1: PSMA1 transfected lysate (30.2kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PSMA1 on HeLa cell. [antibody concentration 60ug/ml])

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to PSMA1 on HeLa cell. [antibody concentration 60ug/ml])

Western Blot (WB)

(Western blot analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Product Categories/Family for anti-PSMA1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
30,239 Da
NCBI Official Full Name
Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 1, mRNA
NCBI Official Synonym Full Names
proteasome subunit alpha 1
NCBI Official Symbol
PSMA1
NCBI Official Synonym Symbols
NU; HC2; PROS30; HEL-S-275
NCBI Protein Information
proteasome subunit alpha type-1
Protein Family

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Research Articles on PSMA1

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Product Notes

The PSMA1 (Catalog #AAA6149089) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSMA1 (Proteasome Subunit alpha Type-1, 30kD Prosomal Protein, PROS-30, Macropain Subunit C2, Multicatalytic Endopeptidase Complex Subunit C2, Proteasome Component C2, Proteasome nu Chain, HC2, NU, PROS30, PSC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). IF: 60ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PSMA1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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