Mouse anti-Human PRTN3 / Proteinase 3 Monoclonal Antibody | anti-PRTN3 antibody

Anti-PRTN3 / Proteinase 3 Antibody (aa28-248, clone 5B8) IHC-plus

Cat. Num. AAA249449

• Gene Names: PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: PRTN3 / Proteinase 3; Monoclonal Antibody; Anti-PRTN3 / Proteinase 3 Antibody (aa28-248; clone 5B8) IHC-plus; Mouse Monoclonal [clone 5B8] (IgG2b) to Human PRTN3 / Proteinase 3; Human PRTN3 / Proteinase 3; ACPA; Azurophil granule protein 7; C-ANCA antigen; CANCA; C-ANCA; AGP7; Leukocyte proteinase 3; MBN; Myeloblastin; NP-4; Neutrophil proteinase 4; NP4; p29; PR-3; Proteinase 3; MBT; Wegener autoantigen; PR3; Serine proteinase; anti-PRTN3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 5B8
• Specificity: Human PRTN3 / Proteinase 3
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 256

• Applicable Applications for anti-PRTN3 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC-P (10 ug/ml); Optimal dilution to be determined by the end user.
• Immunogen Species: PRTN3 / Myeloblastin antibody was raised against Human
• Antigen Type: Recombinant protein
• Immunogen: PRTN3 / Myeloblastin antibody was raised against human recombinant protein fragment corresponding to amino acids 28-248 of human PRTN3 (NP_002768) produced in E. coli.
• Epitope: aa28-248
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Avoid freeze-thaw cycles. Stable for 12 months from date of receipt if properly stored.

Immunohistochemistry - Paraffin (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

NCBI and Uniprot Product Information

• NCBI GI #: 71361688
• NCBI GeneID: 5657
• NCBI Accession #: NP_002768.3
• NCBI GenBank Nucleotide #: NM_002777.3
• UniProt Accession #: P24158; P15637; P18078; Q4VB08; Q4VB09; Q6LBM7; Q6LBN2; Q9UD25; Q9UQD8
• NCBI Official Full Name: myeloblastin
• NCBI Official Synonym Full Names: proteinase 3
• NCBI Official Symbol: PRTN3
• NCBI Official Synonym Symbols: MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
• NCBI Protein Information: myeloblastin
• UniProt Protein Name: Myeloblastin
• Protein Family: Myeloblastin
• UniProt Gene Name: PRTN3
• UniProt Synonym Gene Names: MBN; PR-3; PR3; NP-4
• UniProt Entry Name: PRTN3_HUMAN

Uniprot Description

PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Cell cycle regulation; EC 3.4.21.76; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; extracellular matrix; extracellular space; plasma membrane

Molecular Function: enzyme binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: blood coagulation; collagen catabolic process; negative regulation of phagocytosis; phagocytosis; positive regulation of cell proliferation; proteolysis