Typical Testing Data/Standard Curve (for reference only)
Mouse Resistin-like molecule beta ELISA Kit | RELMb elisa kit
Mouse Resistin-like molecule beta ELISA Kit
Gene Names
RETNLB; XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta
Reactivity
Mouse
Synonyms
Resistin-like molecule beta; Mouse Resistin-like molecule beta ELISA Kit; RELMb elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of RELMbeta. No significant cross-reactivity or interference between RELMbeta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RELMbeta and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RELMb elisa kit
Intended Uses: This RELMbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MouseRELMbeta. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: RELMbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RELMbeta antibody and an RELMbeta-HRP conjugate. The assay sample and buffer are incubated together with RELMbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RELMbeta concentration since RELMbeta from samples and RELMbeta-HRP conjugate compete for the anti-RELMbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by RELMbeta from the sample, fewer sites are left to bind RELMbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RELMbeta concentration in each sample is interpolated from this standard curve.
Principle of the Assay: RELMbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RELMbeta antibody and an RELMbeta-HRP conjugate. The assay sample and buffer are incubated together with RELMbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RELMbeta concentration since RELMbeta from samples and RELMbeta-HRP conjugate compete for the anti-RELMbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by RELMbeta from the sample, fewer sites are left to bind RELMbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RELMbeta concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RELMb elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,730 Da
NCBI Official Full Name
resistin-like beta
NCBI Official Synonym Full Names
resistin like beta
NCBI Official Symbol
RETNLB
NCBI Official Synonym Symbols
XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta
NCBI Protein Information
resistin-like beta; found in inflammatory zone 1; colon carcinoma-related gene protein; cysteine-rich secreted protein FIZZ2; cysteine-rich secreted A12-alpha-like protein 1; cysteine-rich secreted protein A12-alpha-like 1; colon and small intestine-specific cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2
UniProt Protein Name
Resistin-like beta
UniProt Gene Name
RETNLB
UniProt Synonym Gene Names
CCRG; FIZZ2; HXCP2; RETNL2; UNQ408/PRO770
UniProt Entry Name
RETNB_HUMAN
Uniprot Description
resistin-like beta: Probable hormone. Belongs to the resistin/FIZZ family.
Chromosomal Location of Human Ortholog: 3q13.1
Cellular Component: extracellular region
Molecular Function: hormone activity
Biological Process: cell proliferation
Research Articles on RELMb
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Product Notes
The Mouse RELMb retnlb (Catalog #AAA746021) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746021 ELISA Kit recognizes Mouse RELMb. It is sometimes possible for the material contained within the vial of "Resistin-like molecule beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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