Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue; Primary Ab: 10ug/ml Mouse Anti-Human ZPI AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Human Protein Z Dependent Protease Inhibitor (ZPI) Monoclonal Antibody | anti-ZPI antibody
Monoclonal Antibody to Protein Z Dependent Protease Inhibitor (ZPI)
Gene Names
SERPINA10; PZI; ZPI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Protein Z Dependent Protease Inhibitor (ZPI); Monoclonal Antibody; Monoclonal Antibody to Protein Z Dependent Protease Inhibitor (ZPI); Enzyme & Kinase; Metabolic pathway; Hematology; anti-ZPI antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C10
Specificity
The antibody is a mouse monoclonal antibody raised against SERPINA10. It has been selected for its ability to recognize SERPINA10 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Pro24~Leu444
Sequence Length
444
Applicable Applications for anti-ZPI antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue; Primary Ab: 10ug/ml Mouse Anti-Human ZPI AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue; Primary Ab: 10ug/ml Mouse Anti-Human ZPI AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein Z-dependent protease inhibitor
NCBI Official Synonym Full Names
serpin family A member 10
NCBI Official Symbol
SERPINA10
NCBI Official Synonym Symbols
PZI; ZPI
NCBI Protein Information
protein Z-dependent protease inhibitor
UniProt Protein Name
Protein Z-dependent protease inhibitor
UniProt Gene Name
SERPINA10
UniProt Synonym Gene Names
ZPI; PZ-dependent protease inhibitor; PZI
UniProt Entry Name
ZPI_HUMAN
NCBI Description
The protein encoded by this gene belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2010]
Uniprot Description
SERPINA10: Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q32.13
Cellular Component: extracellular space
Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity
Biological Process: regulation of acrosome reaction; blood coagulation
Research Articles on ZPI
Similar Products
Product Notes
The ZPI serpina10 (Catalog #AAA2111264) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Protein Z Dependent Protease Inhibitor (ZPI) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Z Dependent Protease Inhibitor (ZPI) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the ZPI serpina10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Pro24~Leu4 44. It is sometimes possible for the material contained within the vial of "Protein Z Dependent Protease Inhibitor (ZPI), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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