Mouse anti-Human Protein Tyrosine Phosphatase Monoclonal Antibody | anti-PTPN6 antibody

Protein Tyrosine Phosphatase, non-receptor type 6

Cat. Num. AAA4381211

• Gene Names: PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Protein Tyrosine Phosphatase; Monoclonal Antibody; non-receptor type 6; PTPN6; SHP1; protein tyrosine phosphatase; HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; 70 kda SHP-1L protein; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C; PTPN6/SHP; anti-PTPN6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: CPTC-PTPN6-2
• Specificity: PTPN6, which is also designated as SHP-1, is expressed primarily in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. It maps to 12p13, a region commonly involved in leukemia-associated chromosomal abnormalities. SHP-1 plays a role in modulating intracellular signaling for various molecules. In T-cell lymphomas and other malignancies, SHP-1 phosphatase expression is lost, by a thus far undetermined mechanism. Preliminary studies suggest that it may be helpful in differentiating mantle/marginal zone lymphomas (SHP-1+) from follicular lymphomas (SHP-1 negative).
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 595

• Applicable Applications for anti-PTPN6 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: For coating, purchase antibody without BSA; WB: 1-2ug/ml
• Positive Control: A431, Jurkat or K562 cell lysates; Lymph node or spleen.
• Immunogen: Recombinant human full-length PTPN6 protein
• Cellular Localization: Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
• Hu-chromosome Location: 12p13.31
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified PTPN6 Mouse Monoclonal Antibody (CPTC-PTPN6-2). Confirmation of Purity and Integrity of Antibody.)

References

1. Kossev, P M, et al. The American Journal of Surgical Pathology, July 2001. 2. Zhang, Q., et al. Proc. Natl. Acad. Sci. U.S.A. (2005)

NCBI and Uniprot Product Information

• NCBI GI #: 18104989
• NCBI GeneID: 5777
• NCBI Accession #: NP_002822.2
• NCBI GenBank Nucleotide #: NP_002822.2
• UniProt Accession #: P29350
• Molecular Weight: 65kDa
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 6 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase non-receptor type 6
• NCBI Official Symbol: PTPN6
• NCBI Official Synonym Symbols: HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 6
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 6
• Protein Family: Protein tyrosine phosphatase
• UniProt Gene Name: PTPN6
• UniProt Synonym Gene Names: HCP; PTP1C; PTP-1C
• UniProt Entry Name: PTN6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. Ref.20

Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure: Monomer. Interacts with MTUS1

By similarity. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT

By similarity. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.29

Subcellular location: Cytoplasm. Nucleus. Note: In neurons, translocates into the nucleus after treatment with angiotensin II

By similarity. Shuttles between the cytoplasm and nucleus via its association with PDPK1. Ref.26 Ref.29

Tissue specificity: Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

Domain: The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation. Ref.31 Ref.33

Post-translational modification: Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation

By similarity. Phosphorylation at Tyr-564 enhances phosphatase activity. Ref.13 Ref.17

Sequence similarities: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.Contains 2 SH2 domains.Contains 1 tyrosine-protein phosphatase domain.