Rat anti-Human Protein Phosphatase 2A, alpha, Catalytic Subunit Monoclonal Antibody | anti-PPP2CA antibody

Protein Phosphatase 2A, alpha, Catalytic Subunit (PP2Aa, PP2A)

Cat. Num. AAA603980

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Protein Phosphatase 2A; alpha; Catalytic Subunit; Monoclonal Antibody; Catalytic Subunit (PP2Aa; PP2A); Anti -Protein Phosphatase 2A; anti-PPP2CA antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8H124
• Specificity: Recognizes human PP2A. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

• Applicable Applications for anti-PPP2CA antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry; Dilution: Immunohistochemistry: This antibody was tested on paraffin-embedded human tissues (liver, small intestine and colon cancer). Recommended working dilution is 8-25ug/mL when using an HRP-DAB detection system. Positive control: normal human liver. Antibody diluent: 0.1 M PBS (pH 7.4) containing 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton X-100, and 0.01% sodium azide. Antigen retrieval: Is not usually required for this target protein, but if low intensity of tissue staining is observed, heat-induced epitope retrieval of PP2A antigen can be done. Staining pattern: Many PP2A-immunoreactive hepatocytes can be found in normal human liver. PP2A staining can be seen in the cytoplasm and has punctate appearance.
• Immunogen: E. coli-derived recombinant human PP2A-alpha catalytic subunit (aa209-309)
• Hybridoma: Produced from a hybridoma elicited from a rat immunized with amino acids 209-309 of purified, E. coli-derived recombinant human PP2A-alpha catalytic subunit (Accession # M60483).
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PPP2CA antibody

PP2A, also known as PPP2CA and PP2CA, is a serine/threonine phosphatase.

Product Categories/Family for anti-PPP2CA antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 288411
• NCBI GeneID: 282320
• UniProt Accession #: P67774; P05323; P13197; A6QLI7
• Molecular Weight: 35,594 Da
• NCBI Official Full Name: protein phosphatase-2A
• NCBI Official Synonym Full Names: protein phosphatase 2, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP2CA
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha
• UniProt Entry Name: PP2AA_BOVIN

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; meiotic cell cycle; regulation of transcription, DNA-dependent; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; mRNA catabolic process, nonsense-mediated decay; negative regulation of tyrosine phosphorylation of Stat3 protein; regulation of protein catabolic process; gene expression; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process