Mouse anti-Human Properdin Monoclonal Antibody | anti-CFP antibody

Properdin, Human, mAb 3A3E1

Cat. Num. AAA584820

• Gene Names: CFP; BFD; PFC; PFD; PROPERDIN
• Reactivity: Human
• Applications: Western Blot
• Synonyms: Properdin; Monoclonal Antibody; Human; mAb 3A3E1; anti-CFP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: 1 ml (100 mug/ml) 0.2 mum protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 469

• Applicable Applications for anti-CFP antibody: Functional Studies, Immunoassay, Western Blot (WB)
• Preparation and Storage: Should be stored at 4 degree C.; Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-CFP antibody

Monoclonal antibody HM2355 3A3E1 recognizes human properdin, also called complement factor P. The complement system is the first line of defense against pathogens and facilitates elimination of apoptotic and damaged cells. Positive regulator plasma protein properdin is critical for the alternative pathway of complement. It is a single-chain glycoprotein (ca 53kDa) consisting of six TSR sequences. In the blood it exists as a mixture of preferably head-to-tail trimers, but also dimers and tetramers. The protein is produced by leukocytes, like activated neutrophils monocytes and T-lymphocytes, but also by eg. stressed endothelial cells.
Properdin can both initiate and positively regulate the alternative pathway activity together with C3 and factors B, D, I and H. It binds to C3b where It stabilizes the labile C3bBb convertase which is deposited on immune complexes or foreign surfaces. Thereby enhancing the AP by stimulation of amplification of C3bBb-convertase formation in competition with catabolism of C3b by factor I, which uses factor H as a cofactor. The local amplification process leads to the creation of the alternative pathway C5 convertase, C3bBb3b, and initiates the terminal pathway of complement activation. The alternative pathway may account for ca 80% of the terminal pathway activity. Properdin has also been shown to directly limit factor H activity.
Recent studies show that properdin is also a pattern-recognition receptor (PRR) able to bind directly to microbial surfaces, apoptotic and necrotic cells (dangerous nonself and altered self). Inappropriate activation or dysregulation of the alternative pathway is a critical factor in development of several autoimmune conditions. Targets opsonized with properdin are labeled for clearance by scavenger cells, even without complement. This makes it a potential therapeutic target in diseases. Recent studies has shown renewed interest in the evaluating role of properdin in disease pathogenesis, like Asthma, arthritis, septic shock, AMD and C3 glomerulopathy.
Antibody 3A3E1 can be used in ELISA, western blotting (only non-reduced) and as antibody for functional studies inhibiting the alternative pathway of complement, like the standard erythrocyte AP50 assay.

Product Categories/Family for anti-CFP antibody

Autoimmunity; Nephrology

References

1. Blatt A et al; Properdin-mediated C5a production enhances stable binding of platelets to granulocytes in human whole blood. J Immunol 2016; 196: 4671.

NCBI and Uniprot Product Information

• NCBI GI #: 223671861
• NCBI GeneID: 5199
• NCBI Accession #: NP_001138724.1
• NCBI GenBank Nucleotide #: NM_001145252.1
• UniProt Accession #: P27918; O15134; O15135; O15136; O75826
• Molecular Weight: 51,276 Da
• NCBI Official Full Name: properdin
• NCBI Official Synonym Full Names: complement factor properdin
• NCBI Official Symbol: CFP
• NCBI Official Synonym Symbols: BFD; PFC; PFD; PROPERDIN
• NCBI Protein Information: properdin
• UniProt Protein Name: Properdin
• Protein Family: Properdin
• UniProt Gene Name: CFP
• UniProt Synonym Gene Names: PFC

NCBI Description

This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009]

Uniprot Description

A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.