Proinsulin Monoclonal Antibody | anti-INS antibody

MAb to Proinsulin

Cat. Num. AAA310707

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Applications: ELISA, Immunohistochemistry, immunoassay, Radioimmunoassay
• Purity: >90% pure (SDS-PAGE). Protein A chromatography
• Synonyms: Proinsulin; Monoclonal Antibody; MAb to Proinsulin; Monoclonal Antibody to Human Proinsulin; anti-INS antibody

• Host: Mouse. Hybridization of X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 3A1
• Specificity: Proinsulin; Reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule. Does not cross-react with human, bovine, porcine insulin, bovine and porcine proinsulin, and human C-peptide.
• Purity/Purification: >90% pure (SDS-PAGE). Protein A chromatography
• Form/Format: Purified, Liquid
• Concentration: 5.7mg/ml (OD280nm, E^0.1% = 1.4) (varies by lot)
• Sequence Length: 110

• Applicable Applications for anti-INS antibody: EIA/ELISA, Immunohistochemistry, Immunoassay Antibody Pairs, Radioimmunoassay
• Application Notes: Detection of human proinsulin in two-site enzyme immunoassay. Can be used in immunohistochemistry on frozen sections. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested
• Immunogen: Purified human proinsulin.
• Affinity Constant: 1.1 x 10^-8 M
• Buffer: PBS, pH 7.4
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Store at 2 to 8 degree C.

Product Categories/Family for anti-INS antibody

Monoclonal Antibodies to Hormones and Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 4557671
• NCBI GeneID: 3630
• NCBI Accession #: NP_000198.1
• NCBI GenBank Nucleotide #: NM_000207.2
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin preproprotein
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; negative regulation of protein oligomerization; positive regulation of glucose import; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; negative regulation of vasodilation; negative regulation of lipid catabolic process; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cell migration; positive regulation of cytokine secretion

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10